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. 2025 Feb 14;41(2):72.
doi: 10.1007/s11274-025-04252-7.

A psychrotolerant extracellular phosphatase from Krossfjorden sediment bacterium Bacillus cereus KR_O9: purification and functional characterization

E S Lisintta  1 , Annie George  1 , Sona Gafoor  1 , S Vishnupriya  2 , Jesmi Yousuf  2 , Bhavya Kachiprath  2   3 , A K Valsa  4 , Ally C Antony  5 , P A Aneesa  6   7 , A A Mohamed Hatha  8   9
Affiliations

A psychrotolerant extracellular phosphatase from Krossfjorden sediment bacterium Bacillus cereus KR_O9: purification and functional characterization

E S Lisintta et al. World J Microbiol Biotechnol. .

Abstract

This study reports the identification of a phosphatase-producing psychrotolerant bacterium, Bacillus cereus KR_O9, isolated from Arctic Krossfjorden sediments. The extracellular KR_O9 phosphatase was purified to homogeneity through acetone precipitation and ion exchange chromatography, achieving a specific activity of 12.51 U mg⁻1 min⁻1, a 17.3-fold purification, and a 64.94% yield. The molecular mass of the enzyme was estimated as 37 kDa by SDS-PAGE. Km, and Vmax for KR_O9 phosphatase towards substrate p-NPP were determined as 0.033 M L-1, 1.67 mg-1 min-1. The enzyme exhibited optimal activity at 40 °C and pH 6.0, with stability up to ≤ 40 °C and at pH levels ≤ 7.0, highlighting its thermolability and sensitivity to alkaline pH. At 10 mM level, Cu2+, Hg2+, Co2+, Pb2+, urea, EDTA, and GSH significantly stimulated the enzyme, and Ca2+, Zn2+, Mn2+, SDS, and CTAB were, strong inhibitors. The kinetic efficiency, resilience to low to neutral pH, and tolerance to various metal ions, including heavy metals, underscore the potential of B. cereus KR_O9 phosphatase for bioremediation and biofertilizer applications, paving the way for industrial exploration.

Keywords: Arctic; Catalytic efficiency; Characterization; Phosphatases; Psychrotrophs; Purification.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests.

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