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. 2025 Jan 31:2025:10.17912/micropub.biology.001440.
doi: 10.17912/micropub.biology.001440. eCollection 2025.

Antibiotic production by soil bacteria under aerobic and micro-oxic conditions

Cierra Paaaina-Daquioag #  1 , Disha Byadarahalli Mohan Kumar #  1 , Debra R Kerr  1 , Leonardo Romero  1 , Kurt M Regner  1
Affiliations

Antibiotic production by soil bacteria under aerobic and micro-oxic conditions

Cierra Paaaina-Daquioag et al. MicroPubl Biol. .

Abstract

Antimicrobial resistance presents a significant global challenge, undermining the effectiveness of antibiotic therapies and complicating disease management. The origin and spread of antibiotic-resistance genes outpaces the antibiotic discovery process, highlighting an urgent need for new approaches. This study investigated the production of antibiotics by soil bacteria under aerobic and micro-oxic conditions as part of a course-based research experience designed to introduce undergraduates to the global antibiotic resistance crisis. Significant differences in the diameters of the zones of inhibition against three tester strains were observed under differing oxygen concentrations. Soil isolates were identified with 16S rRNA sequence analysis.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. <b>Secondary Antibiosis Screen and Isolate Identification</b>
Figure 1. Secondary Antibiosis Screen and Isolate Identification
(A) Secondary antibiosis screen: soil isolates were patched over a lawn of a single tester strain ( B. subtilis, E. coli, or M. smegmatis ), then incubated under aerobic (top) or micro-oxic (bottom) conditions. (B) Statistically significant differences in the inhibition of tester strains by soil isolates when incubated in aerobic (pink) vs micro-oxic (blue) conditions. Values represent the mean of three replicates with error bars representing SEM (two-sample t-test, p<0.05). (C) Identification of the soil isolates using blastn and SILVA rRNA analysis.
See this image and copyright information in PMC

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