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. 2025 Feb 15;39(2):187-192.
doi: 10.7507/1002-1892.202409094.

[Effect of autologous osteochondral tissue and periosteum transplantation on tendon-bone healing of rotator cuff in rabbits]

[Article in Chinese]
Sen Fang  1 , Mingtao Zhang  1 , Xudong Yang  1 , Cairang Daoji  1 , Mingchun Li  1 , Zhixuan Nian  1 , Junwen Liang  1 , Xiangdong Yun  1   2
Affiliations

[Effect of autologous osteochondral tissue and periosteum transplantation on tendon-bone healing of rotator cuff in rabbits]

[Article in Chinese]
Sen Fang et al. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. .

Abstract
in English, Chinese

Objective: To investigate the effect of autologous osteochondral tissue and periosteum transplantation on tendon-bone healing of rotator cuff in rabbits.

Methods: Twenty-four male New Zealand white rabbits were randomly divided into autologous osteochondral tissue and periosteum transplantation group (experimental group, n=12) and simple suture group (control group, n=12). Both groups were subjected to acute supraspinatus tendon injury and repaired with corresponding techniques. At 4, 8, and 12 weeks after operation, 4 specimens from each group were taken from the right shoulder joint for histological examination (HE staining, Masson staining, and Safranin O-fast green staining), and the left shoulder was subjected to biomechanical tests (maximum tensile load and stiffness).

Results: Both groups of animals survived until the completion of the experiment after operation. At 4 weeks after operation, both groups showed less collagen fibers and disorder at the tendon-bone junction. At 8 weeks, both groups showed reduced inflammation at the tendon-bone junction, with more organized and denser collagen fibers and chondrocytes. The experimental group showed better results than the control group. At 12 weeks, the experimental group showed typical tendon-bone transition structure, with increased generation of collagen fibers and chondrocytes, and the larger cartilage staining area. Both groups showed an increase in maximum tensile load and stiffness over time ( P<0.05). The stiffness at 4 weeks and the maximum tensile load at 4, 8, and 12 weeks in the experimental group were superior to control group, and the differences were significant ( P<0.05). There was no significant difference in stiffness at 8, 12 weeks between the two groups ( P>0.05).

Conclusion: Autologous osteochondral tissue and periosteum transplantation can effectively promote the fiber and cartilage regeneration at the tendon-bone junction of rotator cuff and improve the biomechanical effect of shoulder joint in rabbits.

目的: 探讨自体骨软骨联合骨膜移植对兔肩袖腱-骨愈合的作用。.

方法: 将24只雄性新西兰大白兔随机分为自体骨软骨联合骨膜移植组（实验组）和单纯缝合组（对照组），每组12只。两组制备双侧肩关节急性冈上肌肌腱损伤模型后，采用对应方法治疗。术后观察动物一般情况，于4、8、12周各取4只肩关节标本行组织学观察（HE染色、Masson染色、番红O-固绿染色）及生物力学测试（最大拉伸载荷、刚度）。.

结果: 术后两组动物均存活至实验完成。组织学观察示，术后4周两组腱-骨处胶原纤维少且紊乱；8周时两组腱-骨处炎症细胞减少，胶原纤维和软骨细胞排列更有序、致密，其中实验组优于对照组；12周时实验组出现典型腱-骨过渡结构，胶原纤维和软骨细胞生成增多，且实验组软骨染色范围更大。生物力学测试示，两组最大拉伸载荷及刚度均随时间延长而持续增加，3个时间点间比较差异均有统计学意义（ P<0.05）。实验组术后4周刚度及4、8、12周最大拉伸载荷高于对照组，差异有统计学意义（ P<0.05）；实验组术后8、12周刚度与对照组比较差异无统计学意义（ P>0.05）。.

结论: 自体骨软骨联合骨膜移植可有效促进兔肩袖腱-骨交界处纤维和软骨再生，改善肩关节生物力学效果。.

Keywords: Rotator cuff injury; autogenous transplantation; osteochondral tissue; periosteum; rabbit; tendon-bone healing.

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Conflict of interest statement

利益冲突 在课题研究和文章撰写过程中不存在利益冲突；研究经费没有影响文章观点和对研究数据客观结果的统计分析及其报道

Figures

图 1
图 1
Schematic diagram of repairing acute supraspinatus tendon injury with autologous osteochondral tissue and periosteum transplantation 自体骨软骨联合骨膜修复兔急性冈上肌肌腱损伤示意图
图 2
图 2
Biomechanical test 生物力学测试
图 3
图 3
Histological observation of control group (top) and experimental group (bottom) (×100) 对照组（上）及实验组（下）组织学观察（×100）
See this image and copyright information in PMC

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