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[Preprint]. 2025 Feb 4:2025.01.31.635965.
doi: 10.1101/2025.01.31.635965.

High-Throughput Antibody Neutralization Screening in Massively Parallel Droplet Arrays

Matias Gutierrez-Gonzalez, Ahmed S Fahad, Cyrille L Delley, Cheng-Yu Chung, Shuyan Jin, Nicoleen Boyle, Matheus Oliveira de Souza, Azady Pirhanov, Nicolas M S Galvez, Camila T França, Samantha Marglous, Evan Bhagat, Donovan Vincent, Daniel Neumeier, Yi Cao, Nicole Doria-Rose, Sai T Reddy, Aaron G Schmidt, Alejandro B Balazs, Adam R Abate, Brandon J DeKosky

High-Throughput Antibody Neutralization Screening in Massively Parallel Droplet Arrays

Matias Gutierrez-Gonzalez et al. bioRxiv. .

Abstract

Neutralizing antibodies provide rapid immune defense against infectious diseases, but are difficult to discover at scale because neutralization assays require live reporter cells and soluble monoclonal antibodies. Here we report Droplet Reporter Cell Testing for Neutralization (DrReCT-Neutralization) to screen antibody gene libraries for their ability to neutralize viral infections. We established the necessary engineered cell lines and validated the DrReCT screening platform using synthetic oligoclonal libraries, followed by an example discovery campaign that demonstrated scalable functional antibody data collection against viral diseases.

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