Future Directions of the Prokaryotic Chromosome Field

E A Abbondanzieri¹, A B Badrinarayanan², D Barillà³, S D Bell⁴, F Blombach⁵, J Y Bouet⁶, S Bulgheresi⁷, Q A D Cao⁸, R T Dame⁹, C Dekker¹⁰, M Demuysere¹¹, O Espéli¹², P C M Fogg¹³, P L Freddolino¹⁴, M Ganji¹⁵, T M Gerson¹⁶, D C Grainger¹⁷, L W Hamoen¹⁸, J Harju¹⁹, A Hocher²⁰, C M Hustmyer²¹, J K Kaljevic²², M K Karney¹⁶, N Kleckner²³, G Laloux²², R Landick²¹, V S Lioy²⁴, W L Liu⁸, C L Liu²⁵, J Mäkelä²⁶, A S Meyer¹, A Noy¹³, M P Pineau²⁷, K Premrajka²⁸, L R Racki²⁹, F-Z M Rashid³⁰, K Schnetz³¹, S Schwab³⁰, M Tišma¹⁰, A I van der Sijs³², T van Heesch³³, R van Raaphorst²², J Vreede³³, A W Walker¹, J-C Walter³⁴, S C Weber^{35

36}, P A Wiggins³⁷, H J Wing¹⁶, J Xiao³⁸, Z Zhang⁸

Affiliations

¹ Department of Biology, University of Rochester, Rochester, New York, USA.
² NCBS, Bengaluru, India.
³ Department of Biology, University of York, York, UK.
⁴ Indiana University, Bloomington, Indiana, USA.
⁵ University College, London, UK.
⁶ LMGM-CBI, CNRS, University of Toulouse Paul Sabatier, Toulouse, France.
⁷ Department of Functional and Evolutionary Ecology, Environmental Cell Biology Group, University of Vienna, Vienna, Austria.
⁸ Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Shenzhen, China.
⁹ Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.
¹⁰ Kavli Institute of Nanoscience Delft, Delft, the Netherlands.
¹¹ Molecular Microbiology and Structural Biochemistry, CNRS/University of Lyon 1, Lyon, France.
¹² Collége de France, Paris, France.
¹³ University of York, York, UK.
¹⁴ University of Michigan, Ann Arbor, Michigan, USA.
¹⁵ Indian Institute of Science, Bangalore, India.
¹⁶ University of Nevada-Las Vegas, Las Vegas, Nevada, USA.
¹⁷ University of Birmingham, Birmingham, UK.
¹⁸ University of Amsterdam, Amsterdam, the Netherlands.
¹⁹ Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
²⁰ Medical Research Council London Institute of Medical Sciences, London, UK.
²¹ Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
²² de Duve Institute, UCLouvain, Brussels, Brussels, Belgium.
²³ Harvard University, Cambridge, Massachusetts, USA.
²⁴ CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, France.
²⁵ Chinese Academy of Sciences, China.
²⁶ Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
²⁷ Microbiology, Adaptation and Pathogenesis, UMR5240, INSA, Lyon, France.
²⁸ University of Kiel, Kiel, Germany.
²⁹ University of Kiel, USA.
³⁰ Leiden University, Leiden, the Netherlands.
³¹ Institute for Genetics, University of Cologne, Cologne, Germany.
³² Universiteit Utrecht, Utrecht, the Netherlands.
³³ Van't Hoff Institute for Molecular Sciences, University of Amsterdam, the Netherlands.
³⁴ Laboratory Charles Coulomb (L2C), CNRS & Montpellier Université, Montpellier, France.
³⁵ Department of Biology, McGill University, Montreal, Quebec, Canada.
³⁶ Department of Physics, McGill University, Montreal, Quebec, Canada.
³⁷ University of Washington, Seattle, Washington, USA.
³⁸ Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

PMID: 39977301
DOI: 10.1111/mmi.15347

Review

Future Directions of the Prokaryotic Chromosome Field

E A Abbondanzieri et al. Mol Microbiol. 2025 Feb.

. 2025 Feb;123(2):89-100.

doi: 10.1111/mmi.15347.

Authors

Affiliations

¹ Department of Biology, University of Rochester, Rochester, New York, USA.
² NCBS, Bengaluru, India.
³ Department of Biology, University of York, York, UK.
⁴ Indiana University, Bloomington, Indiana, USA.
⁵ University College, London, UK.
⁶ LMGM-CBI, CNRS, University of Toulouse Paul Sabatier, Toulouse, France.
⁷ Department of Functional and Evolutionary Ecology, Environmental Cell Biology Group, University of Vienna, Vienna, Austria.
⁸ Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Shenzhen, China.
⁹ Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.
¹⁰ Kavli Institute of Nanoscience Delft, Delft, the Netherlands.
¹¹ Molecular Microbiology and Structural Biochemistry, CNRS/University of Lyon 1, Lyon, France.
¹² Collége de France, Paris, France.
¹³ University of York, York, UK.
¹⁴ University of Michigan, Ann Arbor, Michigan, USA.
¹⁵ Indian Institute of Science, Bangalore, India.
¹⁶ University of Nevada-Las Vegas, Las Vegas, Nevada, USA.
¹⁷ University of Birmingham, Birmingham, UK.
¹⁸ University of Amsterdam, Amsterdam, the Netherlands.
¹⁹ Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
²⁰ Medical Research Council London Institute of Medical Sciences, London, UK.
²¹ Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
²² de Duve Institute, UCLouvain, Brussels, Brussels, Belgium.
²³ Harvard University, Cambridge, Massachusetts, USA.
²⁴ CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, France.
²⁵ Chinese Academy of Sciences, China.
²⁶ Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
²⁷ Microbiology, Adaptation and Pathogenesis, UMR5240, INSA, Lyon, France.
²⁸ University of Kiel, Kiel, Germany.
²⁹ University of Kiel, USA.
³⁰ Leiden University, Leiden, the Netherlands.
³¹ Institute for Genetics, University of Cologne, Cologne, Germany.
³² Universiteit Utrecht, Utrecht, the Netherlands.
³³ Van't Hoff Institute for Molecular Sciences, University of Amsterdam, the Netherlands.
³⁴ Laboratory Charles Coulomb (L2C), CNRS & Montpellier Université, Montpellier, France.
³⁵ Department of Biology, McGill University, Montreal, Quebec, Canada.
³⁶ Department of Physics, McGill University, Montreal, Quebec, Canada.
³⁷ University of Washington, Seattle, Washington, USA.
³⁸ Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

PMID: 39977301
DOI: 10.1111/mmi.15347

Abstract

In September 2023, the Biology and Physics of Prokaryotic Chromosomes meeting ran at the Lorentz Center in Leiden, The Netherlands. As part of the workshop, those in attendance developed a series of discussion points centered around current challenges for the field, how these might be addressed, and how the field is likely to develop over the next 10 years. The Lorentz Center staff facilitated these discussions via tools aimed at optimizing productive interactions. This Perspective article is a summary of these discussions and reflects the state-of-the-art of the field. It is expected to be of help to colleagues in advancing their own research related to prokaryotic chromosomes and inspiring novel interdisciplinary collaborations. This forward-looking perspective highlights the open questions driving current research and builds on the impressive recent progress in these areas as represented by the accompanying reviews, perspectives, and research articles in this issue. These articles underline the multi-disciplinary nature of the field, the multiple length scales at which chromatin is studied in vitro and in and highlight the differences and similarities of bacterial and archaeal chromatin and chromatin-associated processes.

Keywords: archaeal chromatin; archaeal chromosome; bacterial chromatin; bacterial chromosome; nucleoid.

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References

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Future Directions of the Prokaryotic Chromosome Field

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Future Directions of the Prokaryotic Chromosome Field

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