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. 2025 Feb 7;197(2):kiaf057.
doi: 10.1093/plphys/kiaf057.

Is GCR1 the GPR157 of plants?

Aditi Gotkhindikar  1 , David Chakravorty  2 , Durba Sengupta  3   4 , Manali Joshi  1 , Sarah M Assmann  2
Affiliations

Is GCR1 the GPR157 of plants?

Aditi Gotkhindikar et al. Plant Physiol. .
No abstract available

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Conflict of interest statement

Conflict of interest statement. None declared.

Figures

Figure 1.
Figure 1.
GPCR-like attributes of AtGCR1. A) Split luciferase (Luc) assays reveal AtGCR1 interaction with the canonical Arabidopsis Gα subunit, GPA1. The GPA1-nLuc + AtGCR1-cLuc combination was compared with a GPA1-nLuc + EV-cLuc parent vector negative control and a GPA1-nLuc + RGS1-cLuc positive control. EV, empty vector; in this case, cLuc lacking a fused partner protein. Numbers above the bars correspond to P values indicating statistically significant differences from the GPA1-nLuc + EV-cLuc parent vector. The assay was performed 3 independent times with similar results. Inset: diagram of the GPA1-nLuc + AtGCR1-cLuc or GPA1-nLuc + RGS1-cLuc split Luc protein–protein interaction assay, with color coding of proteins and Luc fragments consistent between the diagram and graph. See supplementary data for full details of experimental methods. B) Three-dimensional structural model of AtGCR1 coupled with GPA1. The proteins are rendered as cartoons. AtGCR1 is colored in cyan, while GPA1 is colored in orange. All SSEs are labeled. C) Interactions between SSEs of AtGCR1 and GPA1. SSEs of AtGCR1 are colored in cyan, while those of GPA1 are colored in orange. Node diameter is proportional to the number of contacts of that SSE, while edge thickness is proportional to the number of contacts between the 2 SSEs.
Figure 2.
Figure 2.
Comparison of AtGCR1 with GPR157. A) AtGCR1 sequence represented as a snake plot. Residues colored in blue are identical between AtGCR1 and the consensus sequence of GPR157, while those residues colored in orange are strongly similar and those residues colored in yellow are weakly similar. B) Sequence alignment of AtGCR1 with the consensus sequence of GPR157. The N- and C-terminal residues are not shown in A) and B) for clarity.
See this image and copyright information in PMC

References

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