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Published Erratum
. 2025 Mar;107(3):100025.
doi: 10.1016/j.molpha.2025.100025. Epub 2025 Feb 21.

Corrigendum to "Repurposing Treprostinil for Enhancing Hematopoietic Progenitor Cell Transplantation"

No authors listed
Published Erratum

Corrigendum to "Repurposing Treprostinil for Enhancing Hematopoietic Progenitor Cell Transplantation"

No authors listed. Mol Pharmacol. 2025 Mar.
No abstract available

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Figures

Fig. 2
Fig. 2
Pretreatment of murine and human HSPCs with treprostinil and forskolin does neither induce apoptosis nor alters cell cycle progression or differentiation potential. (A, B) Human HSPCs were incubated in the absence (control) and presence of 10 μM treprostinil and 30 μM forskolin (Trep + Fsk) for 1 h. Subsequently, the cells were stained with 7-aminoactinomycin D (7-AAD) and annexin V-PE; the proportion of viable, early apoptotic and dead cells was assessed by flow cytometry. Representative dot plots and the quantification from three independent experiments are shown in panels A and B, respectively. (C, D) Human HSPCs were incubated in the absence (control) and presence of 10 μM treprostinil and 30 μM forskolin (Trep + Fsk) for 1 h and 24 h and subsequently stained with propidium iodide. The distribution of cells along the cell cycle and of dead cells (with sub-2n DNA content) was determined by flow cytometry. Panel C shows the representative original dot plots on the left hand side with the areas used for quantification delineated by rectangles; the corresponding histograms of the cell cycle distribution are depicted on the right hand side. (D) summarizes the data obtained in three independent experiments. No difference in the distribution of cells in G0/1, S and G2 phase was detected between untreated and treated cells (1-way ANOVA). (E, F) Murine HSPCs were isolated form bone marrow, pretreated and resuspended in a methylcellulose medium containing growth factors required for supporting the differentiation and growth of granulo-monocytic colony–forming units (CFU-GM) and of the erythrocyte lineage (BFU-E, burst-forming-unit erythroid). After 10 days the number of colonies was counted under a light microscope and the shape and morphology of colonies was observed. Shown are representative photomicrographs and the quantification of three independent experiments. Data are means ± S.E.M. (n = 3).
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