Complete genome sequence of psychrobacter sp. KFRI-CH2-11: A psychrotolerant bacterium with probiotic, biofortification, and antimicrobial potential for the dairy and meat industries

Abstract

This dataset provides the complete genome sequence of Psychrobacter sp. KFRI-CH2-11, isolated from Korean fermented anchovy, Myeolchi-jeotgal. Genomic analysis identified genes involved in Vitamin B12 biosynthesis, carbohydrate metabolism, CRISPR-Cas defense systems, and antioxidant activity, underscoring the strain's potential for use in food biotechnology. Additional genes linked to antibiotic resistance and bioremediation suggest adaptability in diverse environments, particularly cold-chain storage in the dairy and meat industry. PathogenFinder analysis confirmed the absence of pathogenicity-associated genes, validating the strain's suitability as a probiotic and biofortifying agent in food products.

Keywords: Antibiotic resistance; Bioremediation; CRISPR-Cas; Cold-chain storage; Genome; Probiotics; Psychrobacter; Vitamin B12 synthesis.

Fig. 1

Four additional contigs no.2 to 5 Circular genome map of Psychrobacter sp. KFRI-CH2-11. The outermost ring represents the genome size in Mb. Circular map was drawn by applying contigs 2, 3, 4 and 5 annotation result. Marked characteristics are shown from outside to the center; CDS on forward strand (blue bars), CDS on reverse strand (blue bars), tRNA (green lines), rRNA (purple lines), GC content (green histogram) and GC skew (purple histogram).

Fig. 2

Circos plot of the Psychrobacter sp. KFRI-CH2-11 genome. The outermost rings display coding sequences (CDS) in both the forward and reverse strands, color-coded according to EggNOG functional categories (A-Z). Highlighted target genes include amylase (green), lipase (blue), and protease (red). The positions of tRNA, rRNA, and tmRNA are indicated by distinct colors: tRNA (red), rRNA (blue), and tmRNA (green). The inner rings represent GC skew (green and orange), illustrating guanine-cytosine asymmetry, and GC content (blue), with regions exceeding 50 % GC content marked in red. Internal connecting lines depict relationships between loci corresponding to the same gene site, indicating potential genomic interactions or colocalizations.

Fig. 3

Phylogenetic tree constructed using the Maximum Likelihood method based on 16S rRNA gene sequences. The analysis includes Psychrobacter sp. KFRI-CH2–11 (BOLD) and closely related strains, with 1000 bootstrap replicates. The database used for the analysis is EzBioCloud 16S database (https://www.ezbiocloud.net/) version 20,230,823, and the Psychrobacter sp. sequences included in the bootstrapping process total 45. The bar represents 0.01 substitutions per nucleotide position, and bootstrap values greater than 50 % are shown at the branches.