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. 2025 May 22;42(2):92-99.
doi: 10.4274/tjh.galenos.2025.2024.0424. Epub 2025 Feb 24.

miR-379-5p Inhibited the Proliferation of Acute Myeloid Leukemia Cells Through Negative Regulation of YBX1

Huichao Wu  1 , Lin Zhao  2 , Huanyu Guo  3 , Yingjie Xie  4 , Jianhua Hu  5 , Xinxia Tan  6   7
Affiliations

miR-379-5p Inhibited the Proliferation of Acute Myeloid Leukemia Cells Through Negative Regulation of YBX1

Huichao Wu et al. Turk J Haematol. .

Abstract
in English, Turkish

Objective: Acute myeloid leukemia (AML) highly lethal hematological malignancy that is difficult to treat. This study aimed to clarify the molecular mechanisms of miR-379-5p in AML progression.

Materials and methods: Quantitative real-time polymerase chain reaction was utilized to evaluate miR-379-5p expression levels in AML patients and a control group. A receiver operating characteristic curve was created to assess the clinical predictive value of miR-379-5p in AML, while cell experiments used the CCK-8 assay, flow cytometry, and transwell chambers. Potential target genes of miR-379-5p were predicted by employing online bioinformatics tools, followed by validation using a dual luciferase reporter assay.

Results: miR-379-5p expression was significantly decreased in AML patients and had clinical predictive value for the disease. In AML cell lines, miR-379-5p was downregulated; conversely, the upregulation of miR-379-5p inhibited proliferation, migration, and invasion while promoting apoptosis. Notably, YBX1 was a potential target gene of miR-379-5p and its upregulation reduced the effects of miR-379-5p on AML cell behavior.

Conclusion: miR-379-5p has potential as a biomarker for AML by regulating cell proliferation and apoptosis through the targeting of YBX1.

Amaç: Akut miyeloid lösemi (AML) tedavisi zor olan ve oldukça ölümcül bir hematolojik malignitedir. Bu çalışmada AML progresyonunda miR-379-5p nin moleküler mekanizmalarını netleştirmeyi hedefledik.

Gereç ve yöntemler: AML hastaları ve kontrol grup örneklerinde kantitatif gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak miR-379-5p ekspresyon düzeyleri değerlendirildi. AML’de klinik prediktif miR-379-5p değerini ortaya çıkarabilmek için alıcı taraflı karakteristik bir eğri oluşturuldu. Hücre deneyleri ise CCK-8, akım sitometri ve transwell migrasyon tetkikini kullandı. miR-379-5p’nin potansiyel hedefleri online biyoinformatik tahmin araçları ile öngörülmüştür, peşinden dual lusiferaz raportör yöntemi ile doğrulanmıştır.

Bulgular: AML hastalarında miR-379-5p ekspresyonu belirgin şekilde azalmıştır ve hastalık için klinik prediktif değeri olduğu görülmüştür. AML hücre dizelerinde miR-379-5p azalmıştır, oysa ki artmış düzeyleri proliferasyonu, migrasyonu ve invazyonu inhibe ederek apopitozu tetikler. Özellikle YBX1 miR-379-5p geni potansiyel hedefidir ve artmış seviyeleri miR-379-5p nin AML hücre davranışındaki etkilerini azaltmaktadır.

Sonuç: miR-379-5p YBX1’i hedef alarak hücre proliferasyonu ve apopitozisin düzenlenmesi üzerinden AML için potansiyel bir biyobelirteç olabilir.

Keywords: YBX1; Acute myeloid leukemia; miR-379-5p; Cell proliferation; Apoptosis; Diagnostic significance.

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Conflict of interest statement

Conflict of Interest: No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1
Expression level and diagnostic value of miR-379-5p. A) miR-379-5p was downregulated in acute myeloid leukemia. B) miR-379-5p possesses diagnostic value. C) miR-379-5p was downregulated in acute myeloid leukemia cell lines. ***: p<0.001; AML: acute myeloid leukemia; AUC: area under the curve.
Figure 2
Figure 2
Role of miR-379-5p in HL-60 and THP-1 cells. A, B) After transfection with miR-379-5p mimics, the expression level of miR-379-5p was elevated. C, D) miR-379-5p suppressed proliferation. E, F) miR-379-5p suppressed migration. G, H) miR-379-5p inhibited invasion. I, J) miR-379-5p promoted apoptosis. ***: p<0.001.
Figure 3
Figure 3
The targeting relationship of YBX1 and miR-379-5p. A) The predicted results of target genes of miR-379-5p. B) Targeted binding sites. C) Validation of the targeted relationship. ***: p<0.001.
Figure 4
Figure 4
Regulation of HL-60 and THP-1 cells by the miR-379-5p/YBX1 axis. A, B) Regulation of YBX1 expression. C, D) Effect of miR-379-5p/YBX1 on proliferation ability. E, F) Influence of miR-379-5p/YBX1 on migration. G, H) Influence of miR-379-5p/YBX1 on invasion. I, J) Impact of miR-379-5p/YBX1 on apoptosis. ***: p<0.001.
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