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. 1985 Mar 25;13(6):1905-22.
doi: 10.1093/nar/13.6.1905.

Removal of repeated sequences from hybridisation probes

P G Sealey, P A Whittaker, E M Southern
Free PMC article

Removal of repeated sequences from hybridisation probes

P G Sealey et al. Nucleic Acids Res. .
Free PMC article

Abstract

Pre-reassociation of human clone probes, containing dispersed highly repeated sequences, (e.g. Alu and KpnI families), with a large excess of sonicated total human DNA allows signal from single and low copy number components to be detected in transfer hybridisations. The signal from non-dispersed repeated sequences is reduced to single copy levels. The procedure, which is simple and quick, is illustrated using model combinations of well characterised cloned probes, and is applied to a sample of randomly chosen cosmid clones. A theoretical assessment is presented which may be useful to those wishing to use this procedure.

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