doi: 10.1093/nar/13.6.1905.
Removal of repeated sequences from hybridisation probes
- PMID: 4000947
- PMCID: PMC341124
- DOI: 10.1093/nar/13.6.1905
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Removal of repeated sequences from hybridisation probes
Nucleic Acids Res.
.
Free PMC article
Abstract
Pre-reassociation of human clone probes, containing dispersed highly repeated sequences, (e.g. Alu and KpnI families), with a large excess of sonicated total human DNA allows signal from single and low copy number components to be detected in transfer hybridisations. The signal from non-dispersed repeated sequences is reduced to single copy levels. The procedure, which is simple and quick, is illustrated using model combinations of well characterised cloned probes, and is applied to a sample of randomly chosen cosmid clones. A theoretical assessment is presented which may be useful to those wishing to use this procedure.
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