Effects of alpha-amanitin on the fine structure of adrenal fasciculata cells in the young rat

Abstract

The administration of 0.2 micrograms/g/bw of alpha-amanitin to approximately 20 g rats provoked the following nuclear modifications in rat adrenal fasciculata cells at 60 min: chromatin condensation, nucleolar fragmentation, increase in the number of PCG and clumping of ICG in the center of the nucleus. At longer time intervals (2.5 and 4.5 hr) these alterations were more evident, but at 24 hr the nuclear structure was back to normal with the exception of a persistent increased number of PCG. After injection of 0.75 micrograms/g/bw and at 2.5 hr, there was pulverization of condensed chromatin, fragmentation and partial segregation of the nucleolus with increased density of the fibrillar component. Cytoplasmic alterations were severe including cap-shaped mitochondria with electron-dense matrix surrounding lipid droplets, reduced endoplasmic reticulum of vesicular type and clusters of microvesicles with dense content in the Golgi trans-area. At 24 hr, the nuclear and cytoplasmic morphology returned to normal. These findings are interpreted as the morphological counterpart of a disturbance of extranucleolar and nucleolar RNA synthesis, as well as of lipid and lipoprotein metabolism, brought about by the drug.