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. 2025:2909:31-43.
doi: 10.1007/978-1-0716-4442-3_3.

Immunophenotyping of B-Cell Subpopulations by Flow Cytometry

Michaela Fux  1 , Joseena Iype  2
Affiliations

Immunophenotyping of B-Cell Subpopulations by Flow Cytometry

Michaela Fux et al. Methods Mol Biol. 2025.

Abstract

This chapter introduces a protocol for immunophenotyping B-cell subsets using flow cytometry, a vital technique in both immunology research and clinical practice. Flow cytometry offers detailed insights into B-cell diversity and function, aiding in understanding B-cell development and guiding therapeutic decisions. This chapter delves into critical aspects such as antibody panels, sample preparation, and data analysis, all of which are essential for effectively analyzing B-cell subpopulations in diagnosing conditions like common variable immunodeficiency and monitoring therapeutic outcomes after B-cell depletion. The described protocol ensures accurate quantitative and qualitative determination of total B cells and subpopulations, providing a valuable resource for researchers and clinicians alike.

Keywords: Antibody panels; B-cell depletion; B-cell subsets; Flow cytometry; Immunodeficiency; Immunophenotyping; Monitoring.

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References

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