Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Mar 3;25(1):51.
doi: 10.1007/s10142-025-01565-6.

N-glycosylation of GSTO1 promotes cervical cancer migration and invasion through JAK/STAT3 pathway activation

Affiliations

N-glycosylation of GSTO1 promotes cervical cancer migration and invasion through JAK/STAT3 pathway activation

Panpan Yu et al. Funct Integr Genomics. .

Abstract

Protein glycosylation is strongly associated with tumor progression. Glutathione S-transferase omega 1 (GSTO1) is a member of the glutathione S-transferase family. The significance of GSTO1 N-glycosylation in the progression of cervical cancer (CC) has remained elusive. In this study, we investigated the functional significance of GSTO1 N-glycosylation in CC progression. We employed immunohistochemistry to detect the relative expression of evaluating the link between GSTO1 in CC and benign tissues and the overall survival (OS) and progression-free survival (PFS) in CC patients.In vitro and in vivo experiments to detect CC cell proliferation or metastatic ability after GSTO1 downregulation. NetNGly1.0 Server database predicts potential N-glycosylation modification sites of GSTO1 (Asn55, Asn135, Asn190). Investigating GSTO1 N-glycosylation's function in cellular migration, invasion and epithelial-mesenchymal transition (EMT), we mutated the N-glycosylation sites of GSTO1 through lentivirus-based insertional mutagenesis. Detection of signalling pathways associated with N-glycosylation-modified GSTO1 by enrichment analysis and Western blot. Compared to normal cervical tissue, CC tissue showed significantly higher GSTO1 expression. Further, high GSTO1 levels were a poor predictor of OS and PFS. Both cell and animal experiments suggested that down-regulation of GSTO1 inhibited cell proliferation and metastasis. Glycosylation modification of targeted mutant GSTO1 at positions 55, 135 and 190 significantly inhibits migration and invasion of CC cells. GSTO1 N-glycosylation fixed point mutation inhibits EMT process in CC cells. Mechanistically, N-glycosylated GSTO1 promoted the expression of JAK/STAT3 pathway related markers. GSTO1 N-glycosylation is associated with CC progression and may promote EMT via JAK/STAT3 signaling.

Keywords: Cervical cancer; EMT; GSTO1; JAK/STAT3; N-glycosylation.

PubMed Disclaimer

Conflict of interest statement

Declarations. Ethics approval and consent to participate: This study was approved by the Medical Ethics Committee of the First Affiliated Hospital of Shihezi University (KJ2020-065–01 and A2020-115–01). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.

References

    1. Arbyn M, Weiderpass E, Bruni L, de Sanjosé S, Saraiya M, Ferlay J et al (2020) Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis. Lancet Glob Health 8(2):e191–e20 - PubMed - DOI
    1. Bangarh R, Khatana C, Kaur S, Sharma A, Kaushal A, Siwal S et al (2023) Aberrant protein glycosylation: implications on diagnosis and immunotherapy. Biotechnol Adv 66:108149 - PubMed - DOI
    1. Castle PE, Einstein MH, Sahasrabuddhe VV (2021) Cervical cancer prevention and control in women living with human immunodeficiency virus. CA Cancer J Clin 71(6):505–526 - PubMed - PMC - DOI
    1. Čaval T, Alisson-Silva F, Schwarz F (2023) Roles of glycosylation at the cancer cell surface: opportunities for large scale glycoproteomics. Theranostics 13(8):2605–2615 - PubMed - PMC - DOI
    1. Chen Y, Sun L, Li D et al (2022) Aspirin inhibits carcinogenesis of intestinal mucosal cells in UC mice through inhibiting IL-6/JAK/STAT3 signaling pathway and modulating apoptosis and proliferation. Turk J Gastroenterol 33(9):731–742 - PubMed - PMC - DOI

MeSH terms