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. 2025 Mar-Apr;15(2):395-401.
doi: 10.1016/j.jobcr.2025.02.005. Epub 2025 Feb 17.

Green synthesis of silver nanoparticles using neem and turmeric extract and its antimicrobial activity of plant mediated silver nanoparticles

Rohit Kumar Singh  1 , Deepak Nallaswamy  1 , Shanmugam Rajeshkumar  2 , Sheeja S Varghese  1
Affiliations

Green synthesis of silver nanoparticles using neem and turmeric extract and its antimicrobial activity of plant mediated silver nanoparticles

Rohit Kumar Singh et al. J Oral Biol Craniofac Res. 2025 Mar-Apr.

Abstract

Introduction: The green synthesis of silver nanoparticles has gained attention for being environmentally friendly and cost-effective. This study investigates the synthesis of silver nanoparticles using neem and turmeric extracts, which serve as natural reducing and capping agents, with a focus on characterizing these nanoparticles and assessing their antimicrobial properties against oral pathogens.

Materials and methods: Neem and turmeric extracts were prepared by heating their powdered forms in distilled water, followed by filtration. The extracts were then mixed with a silver nitrate solution, and the reaction was stirred for 24-48 h. The resulting nanoparticles were characterized using UV-Visible spectroscopy, SEM, EDAX, and XRD analysis. The antimicrobial activity of the nanoparticles was tested against four oral pathogens using the agar well diffusion method.

Results: Successful synthesis of silver nanoparticles was confirmed by a color change and characterization analyses. UV-Visible spectroscopy showed a peak at 440 nm, indicating nanoparticle formation. SEM revealed spherical and uniform nanoparticles, while EDAX confirmed the presence of silver. XRD analysis showed the crystalline nature of the nanoparticles, with sizes ranging from 4 nm to 14.81 nm. The nanoparticles exhibited significant antimicrobial activity against Staphylococcus aureus, Streptococcus mutans, and Lactobacillus species, but were less effective against Candida albicans.

Conclusion: The study demonstrates the effectiveness of neem and turmeric extracts in the green synthesis of silver nanoparticles, which exhibited notable antimicrobial activity. This research underscores the potential of plant-mediated synthesis for developing eco-friendly antimicrobial agents.

Keywords: Antimicrobial agent; Eco-friendly; Green synthesis; Silver nanoparticles.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure:1
Figure:1
a & b.
Fig. 2
Fig. 2
SEM image of neem and turmeric mediated silver nanoparticles.
Fig. 3
Fig. 3
EDAX image of neem and turmeric mediated silver nanoparticles.
Fig. 4
Fig. 4
XRD spectra of green synthesized silver nanoparticles.
Fig. 5
Fig. 5
Antimicrobial activity of neem and turmeric mediated silver nanoparticles using agar well diffusion technique. a) Candida albicans b) Lactobacillus c) Staphylococcus aureus d) Streptococcus mutans.
Graph 1
Graph 1
UV–Visible spectra of green synthesized silver nanoparticles. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Graph 2
Graph 2
Antimicrobial activity of silver nanoparticles against oral pathogens.
See this image and copyright information in PMC

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