[Immunodetermination of peptide factors. I. Synthesis of N-alpha-maleoyl-peptide derivatives]

Abstract

A new synthesis of N-maleoyl-omega-amino acids, their use for the introduction of the maleimido group into peptide factors as well as the stability of this group under standard conditions of conventional peptide synthesis are described. The relatively high stability of N-maleoyl-peptide derivatives in aqueous solutions at neutral or slightly acidic pH values as well as the fast addition of thiol compounds to the maleimido group indicate that these peptide derivatives are well suited for the preparation of tracers of peptide factors and of peptide-protein conjugates.