Meta-analysis to determine the effects of supplementation of niacin during the transition or lactation period on performance in dairy cows

Abstract

Niacin, a key component of pyridine nucleotides, plays a vital role in over 200 metabolic reactions, including carbohydrate, lipid, and AA metabolism. This meta-analysis aimed to evaluate the effects of niacin supplementation on lactational performance in dairy cows. We hypothesized that niacin supplementation during the transition or lactation period would enhance the productive performance of dairy cows. A systematic review identified 41 experiments, comprising 128 treatment means and 1,992 cows, which were included in the meta-analysis. Each experiment featured a nonsupplemented control (CON) group and a supplemented group, with niacin provided as either rumen-unprotected niacin (RUPN) or rumen-protected niacin (RPN). Supplementation was initiated during either the transition or lactation period, resulting in the categorization of data into 4 distinct cohorts: cows supplemented with RUPN or RPN during either the transition or lactation period. Cows supplemented with RUPN during the transition (CON; n = 13 treatment means vs. RUPN; n = 14 treatment means) and lactation periods (CON; n = 33 treatment means vs. RUPN; n = 40 treatment means) had average niacin intakes (mean ± SD) of 13.9 ± 10.8 g/d and 9.1 ± 3.5 g/d, respectively. Cows supplemented with RPN during the transition (CON; n = 4 treatment means vs. RPN; n = 4 treatment means) and lactation periods (CON; n = 8 treatment means vs. RPN; n = 12 treatment means) had niacin intakes of 11.7 ± 7.3 g/d and 4.6 ± 2.0 g/d, respectively. Mixed models were used to investigate the linear and quadratic effects of RUPN or RPN. Meta-analytical statistics in STATA estimated the weighted mean difference (WMD) of milk yield for treatment comparisons (CON vs. RUPN and CON vs. RPN). All models included the random effect of experiment and weighting by the inverse of the SEM squared. Supplementation of niacin, whether RUPN or RPN, during the transition period did not improve performance in dairy cows. However, RUPN supplementation during the regular lactation period led to linear increases in DMI, yields of milk, ECM, protein and lactose, and increments of 0.50, 0.90, 0.70, 0.04, and 0.05 kg/d, respectively, were observed when cows were supplemented with 9.1 g/d RUPN. In 38 treatment comparisons from 20 experiments evaluating RUPN supplementation during the lactation period, the mean response indicated that RUPN cows produced 0.83 kg/d more milk than CON cows (WMD = 0.83; 95% CI = 0.35-1.31), with moderate (I² = 61.7%) heterogeneity observed. Similarly, RPN supplementation during the lactation period led to linear increases in DMI and yields of milk, ECM, fat and lactose, with respective increases of 0.60, 1.10, 0.70, 0.06, and 0.05 kg/d when cows were supplemented with 4.7 g/d niacin. Across 12 treatment comparisons from 8 experiments, RPN-supplemented cows produced 0.96 kg/d more milk than CON cows (WMD = 0.96; 95% CI = 0.62-1.31), with no heterogeneity observed. Overall, feeding 9.1 g/d of niacin from RUPN or 4.7 g/d of niacin from RPN during the lactation period improved milk production without improving feed efficiency in dairy cows.

Keywords: milk production; nicotinamide; nicotinic acid; vitamin B(3).