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. 2025 Mar 8;317(1):533.
doi: 10.1007/s00403-025-04018-z.

DNA methylation-based analysis of leukocyte landscape in children with atopic dermatitis

Richie Jeremian  1 , Kaiyang Li  2   3 , Melissa Galati  4 , Melinda Gooderham  5   6   7 , Cathryn Sibbald  8 , Yuka Asai  9 , Vincent Piguet  10   11 , Carolyn S Jack  2   3
Affiliations

DNA methylation-based analysis of leukocyte landscape in children with atopic dermatitis

Richie Jeremian et al. Arch Dermatol Res. .

Abstract

Atopic dermatitis (AD) is the most common chronic, inflammatory skin condition among the pediatric population. There is a need to better characterize the leukocytic landscape of AD for disease stratification, surveillance, and to identify therapeutic targets. We utilized a novel DNA methylation (DNAm)-based algorithm to study a publicly available, epigenome-wide DNAm dataset of pediatric AD patients and healthy subjects. In this pediatric cohort, AD was associated with significantly lower estimated proportions of memory B, memory CD4 T, naïve CD4 T, naïve CD8 T, and NK cells, but higher proportions of eosinophils and Tregs. Patients with lower IgE (< 500 IU/mL) had lower abundance of naïve B cells and higher abundance of eosinophils. Males had higher eosinophil abundance than female patients. This study corroborates existing on data on leukocytic populations in AD and demonstrates a scalable method for leukocyte quantification, that may be expanded to allow for better correlation to clinical presentation.

Keywords: Atopic dermatitis; DNA-methylation; Epigenetics; Leukocytes.

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Conflict of interest statement

Declarations. Patient consent: Not applicable. IRB approval status: Not applicable. Clinical trials registration number: Not applicable. Competing interests: Dr. Vincent Piguet has received honoraria or fees for consulting and/or speaking for AbbVie, Almirall, Celgene, Janssen, Novartis, and Pfizer and has received departmental support for Cardiff University from AbbVie, Almirall, Alliance, Beiersdorf UK Ltd, Biotest, Celgene, Dermal, Eli Lilly, Galderma, Genus Pharma, Globe Micro, Janssen‐Celag, La Roche‐Posay, L’Oreal, LEO Pharma, Meda, MSD, Novartis, Pfizer, Sinclair Pharma, Spirit, Stiefel, Samumed, Thornton Ross, TyPham, and UCB and for University of Toronto from Sanofi.

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