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. 2024 Dec;16(Suppl 5):S4339-S4343.
doi: 10.4103/jpbs.jpbs_526_24. Epub 2025 Jan 30.

Comparative Evaluation of the Impact of Chloride and Fluoride Based Electrolytes on PDL Cells Before and After Electrochemical Dissolution

Hariprasad Ramachandran  1 , Feby Kuriakose  2 , Shiny Joseph  3 , V N Anoop  4 , Balan K Thushar  5
Affiliations

Comparative Evaluation of the Impact of Chloride and Fluoride Based Electrolytes on PDL Cells Before and After Electrochemical Dissolution

Hariprasad Ramachandran et al. J Pharm Bioallied Sci. 2024 Dec.

Abstract

Objective: Most methods for retrieving fractured instruments have consequences that may affect treatment outcomes. A better outcome can be achieved with electrochemical dissolution without mechanical injury.

Materials and methods: Several electrochemical dissolution solutions are being used including artificial saliva, Tyrode's solution, and sodium fluoride (NaF). These electrolytes were prepared and their respective electrochemical characterization was done before conducting the assay. The present in vitro study compares the possible cytotoxic effect and viability of periodontal ligament cells in artificial saliva, Tyrode's solution, and NaF using an MTT assay.

Results: The comparative analyses of the cell viability and toxicity effects of the electrolytes considered are reported. It was observed that the Artificial saliva (before dissolution: 84.10±11.32; after dissolution: 69.1±11.32) and Tyrode's solution (before dissolution: 77.30±4.11; after dissolution: 61.3±4.11) showed higher cell viability percentages and comparatively reduced toxicity levels before and after dissolution compared to NaF (before dissolution: 36.01±5.27; after dissolution: 11.01±5.27). Statistical analysis of the results observed before and after dissolution provided a significant difference (P<0.05).

Conclusions: The artificial saliva and the Tyrode's solution may be considered as a potential electrochemical dissolution solution during the surgery to achieve a better outcome.

Keywords: Artificial saliva; electrolytes; periodontal ligament; sodium fluoride; tyrode’s solution.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
The effect of electrolytes on cellular toxicity and cell viability after dissolution
Figure 2
Figure 2
(a) Roundening of cell morphology before dissolution (NaF). (b) After dissolution with Artificial saliva, cells are still confluent without much change in morphology. (c) After dissolution with Tyrode’s solution, cells are still confluent without much change in morphology
Figure 3
Figure 3
Analysis was conducted before dissolution (a) and after dissolution (b)
See this image and copyright information in PMC

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