Assessment of IL- 35 Gene Polymorphism in Periodontitis Patients with and without Diabetes - A Cross-Sectional Study

Abstract

Aim: To evaluate the IL-35 Gene Polymorphism in Periodontitis subjects with and without type 2 diabetes mellitus.

Methodology: 96 participants were categorized into three groups, Group 1: Systemically and Periodontally healthy individuals, Group 2: Systemically healthy patients with Generalized chronic periodontitis, Group 3: Generalized chronic periodontitis with type 2 diabetes Mellitus. Genomic DNA was extracted from the whole venous blood samples collected from the participants using The PureLink Genomic Kit. The region spanning rs1580809257 and rs1580801731 were amplified using appropriate primer and reaction conditions. Determination of genotypes for each of the polymorphisms assessed using Amplification Refractory Mutation System (ARMS) - based PCR and Restriction Fragment Length Polymorphism (RFLP). The distribution of genotype and allele frequencies for Case and control groups were compared using the Chi-square test. Hardy Weinberg equilibrium is calculated.

Results: The gene and allele frequency were found to be similar in all three groups.

Conclusion: The study reveals that polymorphism of rs1580809257 and rs1580801731 in the IL-35 gene is neither associated with Periodontitis patients nor Periodontitis with Diabetes patients.

Keywords: Allele frequency; gene polymorphism; genotyping; interleukin- 35; periodontitis.

Graph 1

(a) Genotype frequencies among three groups. (b) Allele frequencies among three groups

Graph 2

(a) Genotype frequencies among three groups. (b) Allele frequencies among three groups

Figure 1

(a and b) Insilico analysis utilized the Imutant 2.0 tool to predict protein stability for rs1580809257

Figure 2

(a and b) Insilico analysis utilized the Imutant 2.0 tool to predict protein stability for rs150801731

Figure 3

Polyphen In silico analysis tool to predict protein stability

Figure 4

SIFT In silico analysis tool to predict protein stability