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Review
. 2025 Mar;97(3):e70050.
doi: 10.1002/wer.70050.

Enhancement of aerobic denitrification process on antibiotics removal: Mechanism and efficiency: A review

Progress Mupindu  1 , Yang-Guo Zhao  1   2 , Chao Pan  1 , Yanan Zhang  1 , Jiannan Liu  1
Affiliations
Review

Enhancement of aerobic denitrification process on antibiotics removal: Mechanism and efficiency: A review

Progress Mupindu et al. Water Environ Res. 2025 Mar.

Abstract

Traditionally, the removal of nitrogenous pollutants from wastewater relied on conventional anaerobic denitrification as well as aerobic nitrification and anoxic denitrification. However, anaerobic denitrification is complicated since it requires stringent environmental conditions as well as a large land, therefore, denitrification and nitrification were performed in two separate reactors. Although high pollutant removal efficiency has been achieved via aerobic nitrification and anoxic denitrification, the demerits of this approach include high operational costs. Other traditional nitrogen removal methods include air stripping, reverse osmosis, adsorption, ion exchange, chemical precipitation, advanced oxidation process, and breakpoint chlorination. Traditional nitrogen removal methods are not only complicated but they are also uneconomical due to the high operational costs. Researchers have discovered that denitrification can be carried out by heterotrophic nitrification-aerobic denitrification (HNAD) microorganisms which remove nitrogen in a single aerobic reactor that does not require stringent operating conditions. Despite the significant effort that researchers have put in, there is still little information known about the mechanisms of antibiotic removal during HNAD. This review begins with an update on the current state of knowledge on the removal of nitrogenous pollutants and antibiotics from wastewater by HNAD. The mechanisms of antibiotic removal via HNAD were examined in detail. Followed by, the enhancement of antibiotics removal via co-metabolism and oxidation of sulfamethoxazole (SMX) as well as the response of microbial communities to antibiotic toxicity. Lastly, the conditions favorable for antibiotic biodegradation and mechanisms for nitrogen removal via HNAD were examined. The findings in this review show that co-metabolism and oxidation of SMX were the main antibiotic biodegradation mechanisms, pathways for antibiotic removal by co-metabolism and oxidation of SMX were also proposed in the discussion. This research indicated the potential of aerobic denitrification in the removal of antibiotics from wastewater. Understanding the mechanisms and pathways of antibiotic removal by HNAD helps wastewater engineers and researchers apply the technology more efficiently. PRACTITIONER POINTS: The mechanisms of antibiotic removal via HNAD were examined in detail. Co-metabolism and oxidation of SMX were the main antibiotic biodegradation mechanisms. Pathways for antibiotic removal by co-metabolism and oxidation of SMX were also proposed. Conditions favorable for antibiotic biodegradation were examined. This research indicated the potential of aerobic denitrification in the removal of antibiotics from wastewater.

Keywords: aerobic denitrification; antibiotics; co‐metabolism; heterotrophic nitrification; sulfamethoxazole.

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References

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