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. 1985:59:99-107.

Production in yeast of hepatitis B surface antigen by R-DNA technology

M De Wilde, T Cabezon, N Harford, T Rutgers, E Simoen, F Van Wijnendaele
  • PMID: 4007280

Production in yeast of hepatitis B surface antigen by R-DNA technology

M De Wilde et al. Dev Biol Stand. 1985.

Abstract

Yeast synthesizes the surface antigen protein of Hepatitis B virus when the structural gene is fused to the promoter from the ARG3 gene. Analysis of extracts and total cells shows that the primary translation product can be identified as a poorly antigenic monomer with an estimated molecular weight of 22K. In cell extracts Y-HBsAg is in the form of 20 nm particles which, like serum derived particles, are highly immunogenic in mice and monkeys. Yeast derived surface antigen is thus a viable alternative to the present serum derived HBV vaccines.

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