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Review
. 2025 Feb 28;26(5):2178.
doi: 10.3390/ijms26052178.

Micronutrient Biofortification in Wheat: QTLs, Candidate Genes and Molecular Mechanism

Adnan Nasim  1   2 , Junwei Hao  2 , Faiza Tawab  3 , Ci Jin  2 , Jiamin Zhu  2 , Shuang Luo  2 , Xiaojun Nie  1   2
Affiliations
Review

Micronutrient Biofortification in Wheat: QTLs, Candidate Genes and Molecular Mechanism

Adnan Nasim et al. Int J Mol Sci. .

Abstract

Micronutrient deficiency (hidden hunger) is one of the serious health problems globally, often due to diets dominated by staple foods. Genetic biofortification of a staple like wheat has surfaced as a promising, cost-efficient, and sustainable strategy. Significant genetic diversity exists in wheat and its wild relatives, but the nutritional profile in commercial wheat varieties has inadvertently declined over time, striving for better yield and disease resistance. Substantial efforts have been made to biofortify wheat using conventional and molecular breeding. QTL and genome-wide association studies were conducted, and some of the identified QTLs/marker-trait association (MTAs) for grain micronutrients like Fe have been exploited by MAS. The genetic mechanisms of micronutrient uptake, transport, and storage have also been investigated. Although wheat biofortified varieties are now commercially cultivated in selected regions worldwide, further improvements are needed. This review provides an overview of wheat biofortification, covering breeding efforts, nutritional evaluation methods, nutrient assimilation and bioavailability, and microbial involvement in wheat grain enrichment. Emerging technologies such as non-destructive hyperspectral imaging (HSI)/red, green, and blue (RGB) phenotyping; multi-omics integration; CRISPR-Cas9 alongside genomic selection; and microbial genetics hold promise for advancing biofortification.

Keywords: biofortification; iron; micronutrient deficiency; selenium; wheat; zinc.

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Conflict of interest statement

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Proposed simplified illustration of Fe, Zn, and Se uptake, transport, and sequestration in wheat based on evidence from model species or wheat; question marks show unidentified transporters. PS; phytosiderophore, PSVs; protein storage vacuole; SP = small proteins, Chelators = nicotian.
Figure 2
Figure 2
An infographic illustration of the omics integration with speed breeding and genome editing can help attain wheat biofortification.
See this image and copyright information in PMC

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