Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli
- PMID: 400797
- PMCID: PMC235296
- DOI: 10.1128/jb.130.2.900-910.1977
Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli
Abstract
To determine the region of 16S ribonucleic acid (RNA) at the interface between 30 and 50S ribosomes of Escherichia coli, 30 and 70S ribosomes were treated with T1 ribonuclease (RNase). The accessibility of 16S RNA in the 5' half of the molecule is the same in 30 and 70S ribosomes. The interaction with 50S ribosomes decreases the sensitivity to T1 RNase of an area in the middle of 16S RNA. A large area near the 3' end of 16S RNA is completely protected in 70S ribosomes. The RNA near the 3' end of the molecule and an area of RNA in the middle of the molecule appear to be at the interface between 30 and 50S ribosomes. One site in 16S RNA, 13 to 15 nucleotides from the 3' end, normally inaccessible to T1 RNase in 30S ribosomes, becomes accessible to T1 RNase in 70S ribosomes. This indicates a conformational change at the 3' end of 16S RNA when 30S ribosomes are associated with 50S ribosomes.
Similar articles
-
Action of ribonuclease T1 on 30S ribosomes of Escherichia coli and its role in sequence studies on 16S ribonucleic acid.J Bacteriol. 1973 Dec;116(3):1304-13. doi: 10.1128/jb.116.3.1304-1313.1973. J Bacteriol. 1973. PMID: 4356617 Free PMC article.
-
Comparative surfact structure of 16S ribosomal ribonucleic acid of 30S ribosomes of procaryotic cells.J Bacteriol. 1979 Oct;140(1):131-40. doi: 10.1128/jb.140.1.131-140.1979. J Bacteriol. 1979. PMID: 387717 Free PMC article.
-
Ribonuclease sensitivity of Escherichia coli ribosomes.J Bacteriol. 1966 Oct;92(4):1099-110. doi: 10.1128/jb.92.4.1099-1110.1966. J Bacteriol. 1966. PMID: 5332866 Free PMC article.
-
An RNA core in the 30S ribosomal subunit of Escherichia coli and its structural and functional significance.Cell Biol Int Rep. 1977 Nov;1(6):487-502. doi: 10.1016/0309-1651(77)90086-8. Cell Biol Int Rep. 1977. PMID: 346237 Review.
-
Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids.Microbiol Rev. 1983 Dec;47(4):621-69. doi: 10.1128/mr.47.4.621-669.1983. Microbiol Rev. 1983. PMID: 6363901 Free PMC article. Review. No abstract available.
Cited by
-
Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis.Proc Natl Acad Sci U S A. 1989 Jul;86(13):4927-31. doi: 10.1073/pnas.86.13.4927. Proc Natl Acad Sci U S A. 1989. PMID: 2662189 Free PMC article.
-
Homology of the 3' terminal sequences of the 18S rRNA of Bombyx mori and the 16S rRNA of Escherchia coli.Nucleic Acids Res. 1979 Nov 10;7(5):1109-19. doi: 10.1093/nar/7.5.1109. Nucleic Acids Res. 1979. PMID: 390496 Free PMC article.
-
Long range RNA-RNA interactions in the 30 S ribosomal subunit of E. coli.Nucleic Acids Res. 1985 Jul 11;13(13):4719-38. doi: 10.1093/nar/13.13.4719. Nucleic Acids Res. 1985. PMID: 2410855 Free PMC article.
-
Phylogenetically preserved inter-rRNA base pairs: involvement in ribosomal subunit association.Nucleic Acids Res. 1994 Sep 25;22(19):3936-42. doi: 10.1093/nar/22.19.3936. Nucleic Acids Res. 1994. PMID: 7937116 Free PMC article.
-
Base changes at position 792 of Escherichia coli 16S rRNA affect assembly of 70S ribosomes.Proc Natl Acad Sci U S A. 1990 May;87(10):3700-4. doi: 10.1073/pnas.87.10.3700. Proc Natl Acad Sci U S A. 1990. PMID: 2140191 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
