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. 1977 May;130(2):946-7.
doi: 10.1128/jb.130.2.946-947.1977.

Fusion of yeast spheroplasts

P van Solingen, J B van der Plaat

Fusion of yeast spheroplasts

P van Solingen et al. J Bacteriol. 1977 May.

Abstract

Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa.

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