Cronobacter Surveillance in Minnesota, United States, 2002-2024

Abstract

Cronobacter sakazakii is an opportunistic pathogen associated with bloodstream infections and meningitis in neonates and infants. C. sakazakii isolated from infants under 1 year of age has been reportable in Minnesota since 2003. Invasive Cronobacter infections in infants was recently made notifiable nationally in 2024. Therefore, this summary of over 20 years of reportable surveillance for C. sakazakii from Minnesota infants will inform other jurisdictions. During 2002-2024, 23 C. sakazakii cases in infants, including 8 invasive and 15 non-invasive cases, were identified and confirmed using MALDI-TOF or conventional biochemical methods. Invasive cases were younger (median, 18 days; range, 8 days to 10 months) than non-invasive cases (median, 98 days; range, 7 days to 8 months). Cronobacter isolates from infant (21), child (4), and adult (15) cases were submitted for whole genome sequencing (WGS) to obtain a contextual understanding of potential relatedness. In addition, C. sakazakii isolated from powdered infant formula (PIF) in 2003 was sequenced for comparison. Sequence type (ST) 4 was responsible for the majority of C. sakazakii cases overall (65%) and invasive infant cases (88%). Opened and unopened PIF was cultured from 10 investigations representing both invasive and non-invasive cases. Two (25%) opened PIF samples tested positive for C. sakazakii ST 4 while one (11%) unopened PIF sample tested positive for C. sakazakii ST 4. WGS demonstrated that no clinical cases were associated with a recognized outbreak. Several cases were likely due to contamination of PIF in the home during preparation, highlighting the importance of infant caregiver education to prevent infections.

Keywords: Cronobacter; infants; surveillance.