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. 2025 Feb 27:18:378-383.
doi: 10.1016/j.ibneur.2025.02.016. eCollection 2025 Jun.

Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice

Jeroen Spanoghe  1 , Arne Van Acker  1 , Evelien Carrette  1 , Kristl Vonck  1 , Paul Boon  1 , Robrecht Raedt  1
Affiliations

Effects of intracerebroventricular administration of dimethyl sulfoxide on hippocampal electrophysiology in mice

Jeroen Spanoghe et al. IBRO Neurosci Rep. .

Abstract

Dimethyl sulfoxide (DMSO) is a commonly used solvent in life sciences due to its excellent ability to dissolve compounds with poor water-solubility. Depending on the applied dose, the variety of DMSO's physiological and biological effects may compromise its suitability as a vehicle molecule. Even low concentrations of DMSO are known to affect neuronal excitability in vitro. As in vivo effects have not been studied extensively, this exploratory study investigated the effects of intracerebroventricular (ICV) administration of different DMSO concentrations on hippocampal electrophysiology in mice. Acute recordings of hippocampal evoked potentials (EPs) and electroencephalography (EEG) were performed before and after ICV injection of a 5 µl DMSO solution, with concentrations ranging from 2.5 % to 100 % DMSO. Solutions containing up to 50 % DMSO had no acute effects on hippocampal electrophysiology. Administration of 75 % and 100 % DMSO was found to alter evoked responses, indicating increased excitability. Our results indicate that DMSO can be used as a vehicle in volumes of 5 µl containing concentrations of up to 50 % without affecting acute hippocampal electrophysiological studies in mice. Higher concentrations should be avoided as these affect neuronal excitability.

Keywords: Dimethyl sulfoxide; Electrophysiology; Evoked potentials; Hippocampus.

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Conflict of interest statement

None.

Figures

Fig. 1
Fig. 1
Chemical structure of dimethyl sulfoxide.
Fig. 2
Fig. 2
Effects of ICV injection of different DMSO concentrations on hippocampal evoked potentials. Changes in field excitatory post-synaptic potential (fEPSP) slope and population spike (PS) amplitude, normalized to baseline, plotted over time. Dashed vertical line indicates time of injection. * indicates timepoints where the 100 % DMSO group (red) significantly differs from other groups with p < 0.05.
Fig. 3
Fig. 3
Effects of ICV injection of different DMSO concentrations on hippocampal electroencephalography (EEG). (A) EEG power spectra before (minute 0–20, blue) and 10 min after (minute 30–40, red) ICV injection of the different DMSO concentrations. (B) Change over time of the total EEG power (1–100 Hz) compared to baseline. Dashed vertical line indicates time of injection.
See this image and copyright information in PMC

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