Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1985 May;10(5):591-9.
doi: 10.1007/BF00964398.

The uptake of serotonin and dopamine by homogenates of frozen rat and human brain tissue

A Stenström, L Oreland, J Hardy, P Wester, B Winblad
Comparative Study

The uptake of serotonin and dopamine by homogenates of frozen rat and human brain tissue

A Stenström et al. Neurochem Res. 1985 May.

Abstract

A recently described procedure of freezing and thawing, which allows retention of metabolic and functional integrity, has been applied in the study of serotonin and dopamine uptake into frozen rat and post mortem human frozen tissue. The Km and Vmax for the serotonin uptake into human hypothalamus were estimated to be 0.12 microM and 0.03 nmol/g/min respectively. The Km and Vmax for the dopamine uptake into human putamen were estimated to be 0.28 microM and 0.13 nmol/g/min respectively. The results indicate that the freezing procedure does not affect the uptake sites for these transmitters. The storage time before freezing is however of importance for the Vmax value. The Km value for the uptake, on the other hand, seems to be rather resistant to storage time before freezing.

PubMed Disclaimer

References

    1. J Neurochem. 1981 Jul;37(1):243-6 - PubMed
    1. Pharmacology. 1980;21(2):85-92 - PubMed
    1. J Neurochem. 1983 Mar;40(3):608-14 - PubMed
    1. Brain Res. 1974 Oct 25;79(3):520-3 - PubMed
    1. J Neural Transm. 1979;46(3):253-62 - PubMed

Publication types