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. 2025 Mar 12:9:a25256768.
doi: 10.1055/a-2525-6768. eCollection 2025.

Assessment of the Haemostatic Potential of Platelets Readied for Transfusion

Unwana Emagha  1 , Khawla Yousif  1 , Michelle Duff  1 , Edwina Geraghty  1 , Janice O'Shaughnessy  2 , Philip Murphy  2 , Stefano Verardi  3 , Stephen Marcella  4 , Roy Bentley  5 , Dermot Cox  1
Affiliations

Assessment of the Haemostatic Potential of Platelets Readied for Transfusion

Unwana Emagha et al. TH Open. .
No abstract available

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Conflict of interest statement

Conflict of Interest None declared.

Figures

Fig. 1
Fig. 1
Residual platelets in transfusion bags (<8 days old) were tested for response to agonists (maximum aggregation rate; MAR) using the Sinnowa PL-12 platelet function analyser. The agonists used were thrombin receptor-activating peptide (TRAP 32 μM, n  = 16), arachidonic acid (AA; 0.2 mg/mL, n  = 38), ADP (5 μM, n  = 38; 50 μM, n  = 16), and adrenaline (ADR; 100 μM, n  = 14).
Fig. 2
Fig. 2
Blood was collected from healthy volunteers and PRP was prepared from it ( n  = 6). Both the blood and corresponding PRP were stimulated with ADP (5 and 10 μM) and arachidonic acid (0.2 and 0.4 mg/mL). The response to agonist is presented as maximum aggregation rate (MAR).
Fig. 3
Fig. 3
Blood was collected from healthy volunteers and PRP was prepared ( n  = 6) and stored at room temperature for 72 hours. PRP was stimulated with agonist immediately after preparing PRP ( t  = 0) and over 72 hours. Aggregation response is presented as maximum aggregation rate (MAR). ( A ) PRP stimulated with 5, 10, and 20 μM ADP. ( B ) PRP stimulated with 0.2. 0.4, and 0.8 mg/mL arachidonic acid.
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