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. 2025 Jan 9;87(1):130-137.
doi: 10.1097/MS9.0000000000002826. eCollection 2025 Jan.

Extensive surveillance of mosquitoes and molecular investigation of arboviruses in Central Iran

Fatemeh Abedi-Astaneh  1 , Hedaiatollah R Rad  1 , Hassan Izanlou  1 , Seied A Hosseinalipour  1 , Amir Hamta  1 , Mohammad Eshaghieh  1 , Mahdi Ebrahimi  1 , Mohammad A Ansari-Cheshmeh  1 , Mohammad H Pouriayevali  2 , Mostafa Salehi-Vaziri  2 , Tahmineh Jalali  2 , Asghar Talbalaghi  1 , Ebrahim Abbasi  3
Affiliations

Extensive surveillance of mosquitoes and molecular investigation of arboviruses in Central Iran

Fatemeh Abedi-Astaneh et al. Ann Med Surg (Lond). .

Abstract

Background: Arboviruses are one of the greatest threats to animal and public health. Culicidae family is one of the most important vectors for the transmission of arboviruses in the world. According to the geographical, demographic, and climatic features of Qom city in Iran, it can be a suitable region for vectors and therefore transmission of arboviruses.

Methods: In this study, which was conducted between 2019 and 2020 in different parts of Qom city, 83 414 mosquitoes were collected, and after evaluating the species of mosquitoes based on morphological and molecular detection, the presence of alphaviruses, flaviviruses, and phleboviruses were evaluated using genus-specific Reverse transcription polymerase chain reaction (RT-PCR) assays.

Results: In this study, Culex tarsalis, Culex theileri, and Culex quinquefasciatus were detected, including the first recorded presence of Culex tarsalis in Iran. No infections with alphavirus, flavivirus, or phlebovirus were identified in the collected mosquitoes.

Conclusion: Climatic and weather changes are the basis for the growth and spread of vectors and, consequently, the spread of arboviral diseases, and this issue seems to be very important to the necessity of increasing and continuing entomological and virological studies.

Keywords: Culicidae; Iran; PCR; arboviruses; entomological surveillance; vector.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1.
Figure 1.
Qom province in Iran, the study map.
Figure 2.
Figure 2.
Left: Biogents (BG) trap and Right: CO2 trap.
Figure 3.
Figure 3.
Map of the study area, Qom city, Qom province, Iran, 2018.
Figure 4.
Figure 4.
Culex tarsalis. Cx. tarsalis was not included in the diagnostic keys of Iran. The genus was identified by the entomologist, while species identification was confirmed by the national reference laboratory.
Figure 5.
Figure 5.
Cx. theileri (unknown to Entomologist).
Figure 6.
Figure 6.
Cx. theileri.
See this image and copyright information in PMC

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