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. 2025 Apr;15(4):95.
doi: 10.1007/s13205-025-04228-2. Epub 2025 Mar 21.

Exploration of potent antimicrobial and antioxidant bioactive compounds of selected medicinal plants against drug-resistant pathogens

Basavaraju Marappa  1 , B S Gunashree  1
Affiliations

Exploration of potent antimicrobial and antioxidant bioactive compounds of selected medicinal plants against drug-resistant pathogens

Basavaraju Marappa et al. 3 Biotech. 2025 Apr.

Abstract

Antibiotic resistance is an escalating universal health threat in this modern era, driving the need for innovative therapeutic solutions. Medicinal plants extensively utilized in Ayurveda, offer significant promises as antibacterial agents. This study examines the metabolite composition of methanol crude extracts from Datura metel and Phyllanthus amarus and evaluates their antibacterial and antioxidant properties. The metabolite content of the crude extracts from the leaves of selected plants was investigated and characterized using HPLC and GC-MS techniques. The agar well diffusion method was used to evaluate antibacterial activity against eight pathogenic bacterial strains, and antioxidant activity was measured using the DPPH assay. Among the tested crude extracts, Datura metel exhibited the strongest antibacterial activity against Salmonella typhi, Acinetobacter baumannii, Klebsiella pneumoniae, and methicillin-resistant Staphylococcus aureus (MRSA). Phyllanthus amarus showed notable activity against Salmonella typhi, Acinetobacter baumannii, methicillin-susceptible Staphylococcus aureus (MSSA), and Klebsiella pneumoniae. The extracts exhibited antimicrobial activity against the tested organisms, with the average diameter of the zone of inhibition ranging from 19 to 21 mm and the two leaf extracts exhibited varying degrees of antibacterial activity. In antioxidant assays, the Datura metel extract demonstrated superior activity, with 78.3 ± 2% inhibition and an IC50 value of 40.1 ± 4 µg/mL. These findings highlight Datura metel and Phyllanthus amarus as promising sources of natural antibacterial and antioxidants, offering potential applications in managing infectious diseases.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04228-2.

Keywords: Antimicrobial activity; Bioactive compounds; Leaf extracts; Medicinal plants; Multidrug resistance.

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Conflict of interest statement

Conflict of interestThe authors declared that there is no conflict of interests regarding the publication of paper. We certify that both the authors on the list has read and approved the manuscript. We further attest that the portion of the experiment described in this publication does not involve any human or animal subject.

References

    1. Acharya CK, Das B, Madhu NR, Sau S, De M, Sarkar B (2023) A comprehensive pharmacological appraisal of Indian traditional medicinal plants with anti-diabetic potential. Adv Diabetes Res Manag. 10.1007/978-981-19-0027-3_8
    1. Adegoke AA, Iberi PA, Akinpelu DA et al (2011) Studies on Phytochemical screening and antimicrobial properties of Phyllanthus amarus against multiple antibiotics resistant bacteria. Int J Appl Res Nat Prod 3:6–8
    1. Ahmed M, Sahibzada MUK, Rasheed HM, Khan T, Wahid F, Farooq U (2022) Inhibition of inflammation associated corneal neovascularization by Dalbergia sissoo and Catharanthus roseus leaves extracts in animal model. South Afr J Bot. 10.1016/j.sajb.2022.03.047
    1. Akinmoladun AC, Ibukun EO, Afor E, Obuotor EM, Farombi EO (2007) Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci Res Essays 2:163–166
    1. Alabri THA, Musalami AHSA, Hossain MA, Weli AM, Riyami QA (2014) Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. J King Saud Univ Sci 26:237–243. 10.1016/j.jksus.2013.07.002

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