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. 2025 Feb 28;15(3):383.
doi: 10.3390/life15030383.

Technical and Performance Characteristics Between Different Anti-Mullerian Hormone (AMH) Assay Methods and Antral Follicle Count (AFC) in Malaysian Women with Infertility: A University-Based Centre Cohort

Chong Jie Wen  1 , Mohd Faizal Ahmad  1 , Muhammad Azrai Abu  1 , Nalisa Shamyra Johari  1 , Izzatul Aliaa Badaruddin  2 , Shah Shamsul Azhar  3 , Abdul Kadir Abdul Karim  1
Affiliations

Technical and Performance Characteristics Between Different Anti-Mullerian Hormone (AMH) Assay Methods and Antral Follicle Count (AFC) in Malaysian Women with Infertility: A University-Based Centre Cohort

Chong Jie Wen et al. Life (Basel). .

Abstract

Backgrounds: Evaluating the AMH level and AFC are crucial in infertility practice. Thus, accurate measurement is paramount. Various methods are available and selecting the right process is essential to ensure good reproducibility and precise results. Thus, our study aims to determine the analytical performance of AMH Plus and AFIAS-AMH and the correlation between the two AMH assays and AFC values.

Methods: A prospective study was conducted at the Advanced Reproductive Center, Hospital Canselor Tuanku Mukhriz (HCTM), Faculty of Medicine, National University of Malaysia, to compare the correlation between the AMH assay methods and AFCs. It included 100 patients from Feb 2024 until June 2024.

Results: A total of 100 women with infertility, with a mean age of 35.87 ± 3.92, were included in our study. Our results revealed a strong positive correlation between the two AMH assays, with the comparable performance of AMH Plus and AFIAS-AMH. We also found that the serum AMH evaluation was comparable with the routine AFC assessment.

Conclusions: Our findings indicated that serum AMH levels determine the association between AMH levels and follicle counts and the correlation between AMH concentrations and assays. Our study demonstrated the competent repeatability, acceptable linearity, and laboratory precision of the AFIAS-AMH test and comparative assessment of the AFC and serum AMH evaluation.

Keywords: anti-Mullerian hormone (AMH); antral follicle count (AFC); ovarian reserve.

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Conflict of interest statement

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
The correlation of the results of AFIAS-AMH and AMH Plus.
Figure 2
Figure 2
(A): The correlation between antral follicle count and AMH Plus. (B): The correlation between antral follicle count and AFIAS-AMH.
Figure 2
Figure 2
(A): The correlation between antral follicle count and AMH Plus. (B): The correlation between antral follicle count and AFIAS-AMH.
Figure 3
Figure 3
(A): The correlation between AFIAS-AMH in low ovarian reserve study population. (B): the correlation between AFIAS-AMH in normal ovarian reserve study population. (C): the correlation between AFIAS-AMH in high ovarian reserve study population.
Figure 3
Figure 3
(A): The correlation between AFIAS-AMH in low ovarian reserve study population. (B): the correlation between AFIAS-AMH in normal ovarian reserve study population. (C): the correlation between AFIAS-AMH in high ovarian reserve study population.
See this image and copyright information in PMC

References

    1. Gleicher N., Weghofer A., Barad D.H. Defining ovarian reserve to better understand ovarian aging. Reprod. Biol. Endocrinol. 2011;9:23. doi: 10.1186/1477-7827-9-23. - DOI - PMC - PubMed
    1. Zhang D., Keilty D., Zhang Z.F., Chian R.C. Mitochondria in oocyte aging: Current understanding. Facts Views Vis. Obgyn. 2017;9:29–38. - PMC - PubMed
    1. Wang X., Jin L., Mao Y.-D., Shi J.-Z., Huang R., Jiang Y.-N., Zhang C.-L., Liang X.-Y. Evaluation of ovarian reserve tests and age in the prediction of poor ovarian response to controlled ovarian stimulation—A real-world data analysis of 89,002 patients. Front. Endocrinol. 2021;12:702061. doi: 10.3389/fendo.2021.702061. - DOI - PMC - PubMed
    1. Ferguson J., Hockley J., Rigsby P., Burns C. Establishment of a WHO Reference Reagent for anti-Mullerian hormone. Reprod. Biol. Endocrinol. 2020;18:86. doi: 10.1186/s12958-020-00641-9. - DOI - PMC - PubMed
    1. Cohen-Haguenauer O., Picard J., Mattei M.-G., Serero S., Van Cong N., De Tand M.-F., Guerrier D., Hors-Cayla M.-C., Josso N., Frézal J. Mapping of the gene for anti-Müllerian hormone to the short arm of human chromosome 19. Cytogenet. Genome Res. 1987;44:2–6. doi: 10.1159/000132332. - DOI - PubMed

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