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. 1985 Mar;145(2):393-7.
doi: 10.1016/0003-2697(85)90379-3.

Synthesis, storage, and stability of [4-14C]oxaloacetic acid

M D Hatch, H W Heldt

Synthesis, storage, and stability of [4-14C]oxaloacetic acid

M D Hatch et al. Anal Biochem. 1985 Mar.

Abstract

A simple procedure for preparing [4-14C]oxaloacetic acid based on the reaction between [14C]HCO-3 and phosphoenolpyruvate catalyzed by phosphoenolpyruvate carboxylase is described. A simple method for preparing highly purified phosphoenolpyruvate carboxylase from maize leaves is described and the degradation of oxaloacetate under conditions of varying pH and divalent metal ion concentration is reported on. [4-14C]Oxaloacetic acid is stable for several months in 0.1 M HCl solution at -80 degrees C.

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