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. 1985 Jun;147(2):285-8.
doi: 10.1016/0003-2697(85)90273-8.

Proteins transferred to nitrocellulose for use as immunogens

K A Knudsen

Proteins transferred to nitrocellulose for use as immunogens

K A Knudsen. Anal Biochem. 1985 Jun.

Abstract

Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and were eluted efficiently from the gel by transfer to nitrocellulose paper. The protein-bearing nitrocellulose was solubilized with dimethyl sulfoxide and used as an immunogen in rabbits. Polyclonal antibodies to the proteins were generated.

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