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. 2025 Mar 27;26(4):98.
doi: 10.1208/s12249-025-03085-9.

Harnessing Exercise-Like Benefits of Protonation prone Liposomal Resveratrol in Differentiated Fat Cells: A Proof-of-Concept Study

Nupur Vasdev  1 , Tanisha Gupta  1 , Anoothi Bain  1 , Dnyaneshwar Kalyane  2 , Suryanarayana Polaka  3 , Rakesh Kumar Tekade  4
Affiliations

Harnessing Exercise-Like Benefits of Protonation prone Liposomal Resveratrol in Differentiated Fat Cells: A Proof-of-Concept Study

Nupur Vasdev et al. AAPS PharmSciTech. .

Abstract

Obesity is a significant health issue resulting from a sedentary lifestyle and is linked to numerous other serious conditions, including cancer, diabetes, and cardiovascular diseases. Consequently, resveratrol (RES) is gaining attention as an emerging therapeutic agent due to its exercise-like effects. However, RES's instability and low aqueous solubility have limited its applications. This research report focuses on the loading, solubilization, and sustained delivery of RES using a dendrimer complex loaded liposomal formulation. The safety and efficacy of formulation was studied by performing various assays. The DEN-RES complex loaded liposomes were optimized using a Quality by Design (QbD) approach whereas particle size, PDI and zeta potential were found to be 159.29 ± 0.58 nm, 0.206 ± 0.008, and -7.2 ± 0.14 mV, which followed first-order release kinetics for sustained RES release. The mRNA levels of the SIRT1 and AMPK genes were found to be upregulated by more than two folds, whereas the LIPO-DEN-RES downregulated the mRNA expression of PPARγ in adipocytes. Therefore, the modulation of mRNA levels detected in 3T3-L1 cells post-treatment with the LIPO-DEN-RES validates the formulation's potential in addressing obesity.

Keywords: DEN-RES complex; liposomes; obesity; quality by design; resveratrol.

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Conflict of interest statement

Declarations. Conflict of Interest: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: The work described in this manuscript has been filed for Indian patent at Indian Patent Office (IPO), Mumbai; India Application No.: 202321038004; Date of filing: 02/06/2023.

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