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. 2025 Mar 7;30(1):36-44.
doi: 10.1089/apb.2024.0024. eCollection 2025 Mar.

Biosafety Level 2 Training Programs at Institutions of Higher Learning in the United States

Katie Hall  1 , Betsy Matos  1 , Anne Dombroski-Brokman  1 , Steven Ziegenfuss  1
Affiliations

Biosafety Level 2 Training Programs at Institutions of Higher Learning in the United States

Katie Hall et al. Appl Biosaf. .

Abstract

Introduction: Biosafety Level 2 (BSL-2) training programs were benchmarked at institutions of higher learning in the United States by surveying Biosafety Officers (BSOs). The goal of this survey was to not only compare Institutional Biosafety Committee (IBC) requirements for BSL-2 training but also compare delivery format, length of training, inclusion of a hands-on or interactive component, and requirement for refresher training.

Methods: Survey subjects were drawn from the National Institutes of Health (NIH) registry of BSOs and were limited to BSOs at institutions of higher learning in the United States. Twelve survey questions were developed in this study. The survey was sent to 324 BSOs, of which 108 responded with sufficient data to be included in the analysis.

Results: The survey found that BSL-2 training is required by the institutional and/or IBC policy at 88% of institutions, is optional at 5.6%, and not offered at 6.5%. More than half of the respondents (60.4%) offered BSL-2 training in an on-demand manner. Some institutions (34.5%) include a component of training that is in person. However, this training does not always include hands-on exercises or interactive activities. The survey indicated that most (96.6%) institutions offered BSL-2 training less than three hours in length, and many (58%) institutions required refresher training.

Conclusions: Most institutions of higher learning in the United States, which are registered with the NIH, require BSL-2 training for laboratory personnel involved in research and teaching.

Keywords: Biosafety Level 2; biosafety officer; on-demand; refresher; training.

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