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. 2025 Mar 28;56(2):121.
doi: 10.1007/s10735-025-10404-8.

Localization and function of APC15 during mouse oocyte meiotic progression

Shi-Bin Chao  1   2 , Ren-Ren Zhang  3   4 , Qing-Yuan Sun  5   6   7
Affiliations

Localization and function of APC15 during mouse oocyte meiotic progression

Shi-Bin Chao et al. J Mol Histol. .

Abstract

The Anaphase-Promoting Complex/Cyclosome (APC/C) is a critical regulator of cell cycle progression, with APC15 serving as an essential subunit. While the role of APC15 in mitosis is well characterized, its function during meiosis remains poorly understood. In this study, we investigated the expression, subcellular localization, and potential role of APC15 during mouse oocyte meiotic progression. Using immunofluorescence and confocal microscopy, we observed dynamic changes in APC15 localization throughout meiotic progression. Knockdown of APC15 via siRNA did not affect spindle organization, but led to meiotic arrest at metaphase I (MI) and impaired the removal of BUB3 from kinetochores, suggesting a disruption in Spindle Assembly Checkpoint (SAC) inactivation. Our results highlight the involvement of APC15 in the regulation of SAC and the transition from metaphase to anaphase in oocytes. These findings contribute to our understanding of APC15's role in meiotic regulation and provide insights into its potential impact on maintaining chromosomal stability during oocyte maturation.

Keywords: APC15; Meiosis; Meiotic arrest; Spindle; Spindle assembly checkpoint.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests.

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