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. 1977 Jan 28;195(4276):394-7.
doi: 10.1126/science.401545.

Isolation of eukaryotic DNA fragments containing structural genes and the adjacent sequences

G P Georgiev, Y V Ilyin, A P Ryskov, N A Tchurikov, G N Yenikolopov, V A Gvozdev, E V Ananiev

Isolation of eukaryotic DNA fragments containing structural genes and the adjacent sequences

G P Georgiev et al. Science. .

Abstract

In Drosophila melanogaster structural genes are located close to moderately reiterated sequences. One of the clones obtained contains the DNA related to intercalary heterochromatin of D. melanogaster. These are individual differences in the distribution of genetic material in polytenic chromosomes of different stocks of D. melanogaster. The techniques that allow isolation of DNA fragments containing structural genes at the beginning, in the middle, or the end of the coding strand have been elaborated.

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