Physiol-biochemical, transcriptome, and root microstructure analyses reveal the mechanism of salt shock recovery in sugar beet

Abstract

Soil salinity substantially limits agricultural productivity, necessitating a sound understanding of salt-tolerance mechanisms in key crops for their improved breeding. Despite being a staple sugar crop with strong salt tolerance, sugar beet (Beta vulgaris L.), remains underexplored for its transcriptional responses to salt shock. This study compared the physiological traits, root structure, and full-length transcriptomes of salt-tolerant (T510) and salt-sensitive (S210) sugar beet varieties during stages of osmotic stress (0-24 h) and ionic stress (1-7 d) after incurring salt shock. The results show that T510 recovered faster, maintaining a higher water potential (WP), better osmotic regulation, lower reactive oxygen species (ROS) levels, and a balanced Na⁺/K⁺ ratio. Furthermore, while under osmotic stress, T510 exhibited extensive transcriptional reprogramming to enhance its photosynthetic efficiency and carbon assimilation via the C₄-dicarboxylic acid (C₄) cycle, which compensated for salt shock-induced disruptions to the Calvin-Benson (C₃) cycle. Notably, elevated activity of ascorbate peroxidase (APX) and glutathione S-transferase (GST), driven by greater gene expression, enhanced the scavenging of ROS. In tandem, T510 synthesized more lignin than S210, and adapted its root microstructure to maintain water and nutrient transport functioning in the face of high salinity. Overall, these findings provide insights into the physiological, transcriptomic, and structural adaptations enabling salt tolerance in sugar beet plants, thus offering valuable strategies for strengthening crop resilience through molecular breeding.

Keywords: Beta vulgaris; Lignin; Microstructural; Photosynthesis; Salt shock; Transcriptomic.