Pentamidine inhibition of streptopain attenuates Streptococcus pyogenes virulence

Abstract

The obligate human pathogen Group A Streptococcus (GAS; Streptococcus pyogenes) carries high morbidity and mortality, primarily in impoverished or resource-poor regions. The failure rate of monotherapy with conventional antibiotics is high, and invasive infections by this bacterium frequently require extensive supportive care and surgical intervention. Thus, it is important to find new compounds with adjunctive therapeutic benefits. The conserved secreted protease streptopain (Streptococcal pyrogenic exotoxin B; SpeB) directly contributes to disease pathogenesis by inducing pathological inflammation, degrading tissue, and promoting the evasion of antimicrobial host defense proteins. This study screened 400 diverse off-patent drug and drug-like compounds for inhibitors of streptopain proteolysis. Lead compounds were tested for activity at lower concentrations and anti-virulence activities during in vitro infection. Significant inhibition of streptopain was seen for pentamidine, an anti-protozoal drug approved for the treatment of pneumocystis pneumonia, leishmaniasis, and trypanosomiasis. Streptopain inhibition rendered GAS susceptible to killing by human innate immune cells. These studies identify unexploited molecules as new starting points for drug discovery and a potential for repurposing existing drugs for the treatment of infections by GAS.

Keywords: Streptococcus pyogenes; antibiotics; antiinfective; drug screening; protease; virulence factor.

FIG 1.

Graphical representation of results from the primary screen for each of the 400 Pathogen compounds. (A) Each individual compound was tested at a concentration of 100 μM for hydrolysis of the substrate azo-casein. (B) Distribution of results.

FIG 2.

Activity of candidate compounds against SpeB. Cleavage of sub103 peptide after 30 min incubation with purified SpeB with each compound at the concentration indicated. N=4, error bars represent standard deviations.

FIG 3.

SpeB-inhibitor interactions. Models of the substrate pocket of SpeB and each indicated compound (yellow). Protein surface electrostatics are colored red and blue for negative and positive charges, respectively, and white color represents neutral residues.

FIG 4.

Pentamidine inhibits proteolysis by SpeB. (A-C) Models of the substrate pocket of SpeB and each indicated compound (yellow). Protein surface electrostatics are colored red and blue for negative and positive charges, respectively, and white color represents neutral residues. (D) Cleavage of sub103 peptide after 30 min incubation with purified SpeB was plotted against a range of pentamidine concentrations. N=4, error bars represent standard deviations.

FIG 5.

Pentamidine sensitized GAS to killing by neutrophils. Colony count of GAS (A) or S. aureus (B) after 2 hr infection of human primary neutrophils with 10 μM Pentamidine treatment. *P<0.05; ****P<0.00005 (ANOVA; Tukey post-test). Experiments were performed three times; error bars represent standard deviations.