Artificial intelligence in bacterial diagnostics and antimicrobial susceptibility testing: Current advances and future prospects

Abstract

Recently, artificial intelligence (AI) has emerged as a transformative tool, enhancing the speed, accuracy, and scalability of bacterial diagnostics. This review explores the role of AI in revolutionizing bacterial detection and antimicrobial susceptibility testing (AST) by leveraging machine learning models, including Random Forest, Support Vector Machines (SVM), and deep learning architectures such as Convolutional Neural Networks (CNNs) and transformers. The integration of AI into these methods promises to address the current limitations of traditional techniques, offering a path toward more efficient, accessible, and reliable diagnostic solutions. In particular, AI-based approaches have demonstrated significant potential in resource-limited settings by enabling cost-effective and portable diagnostic solutions, reducing dependency on specialized infrastructure, and facilitating remote bacterial detection through smartphone-integrated platforms and telemedicine applications. This review highlights AI's transformative role in automating data analysis, minimizing human error, and delivering real-time diagnostic results, ultimately improving patient outcomes and optimizing healthcare efficiency. In addition, we not only examine the current advances in machine learning and deep learning but also review their applications in plate counting, mass spectrometry, morphology-based and motion-based microscopic detection, holographic microscopy, colorimetric and fluorescence detection, electrochemical sensors, Raman and Surface-Enhanced Raman Spectroscopy (SERS), and Atomic Force Microscopy (AFM) for bacterial diagnostics and AST. Finally, we discuss the future directions and potential advancements in AI-driven bacterial diagnostics.

Keywords: Antimicrobial susceptibility testing (AST); Artificial intelligence (AI); Bacterial diagnostics; Deep learning; Machine learning.