Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jul 10;188(14):3863-3881.e25.
doi: 10.1016/j.cell.2025.02.037. Epub 2025 Apr 3.

Single-cell spatial transcriptome atlas and whole-brain connectivity of the macaque claustrum

Ying Lei  1 , Yuxuan Liu  2 , Mingli Wang  3 , Nini Yuan  4 , Yujie Hou  5 , Lingjun Ding  6 , Zhiyong Zhu  6 , Zihan Wu  7 , Chao Li  4 , Mingyuan Zheng  4 , Ruiyi Zhang  4 , Ana Rita Ribeiro Gomes  5 , Yuanfang Xu  4 , Zhaoke Luo  5 , Zhen Liu  8 , Qinwen Chai  9 , Pierre Misery  5 , Yanqing Zhong  4 , Xinxiang Song  4 , Camille Lamy  5 , Wei Cui  10 , Qian Yu  4 , Jiao Fang  9 , Yingjie An  4 , Ye Tian  9 , Yiwen Liu  11 , Xing Sun  8 , Ruiqi Wang  4 , Huanhuan Li  4 , Jingjing Song  4 , Xing Tan  4 , He Wang  4 , Shiwen Wang  4 , Ling Han  4 , Yong Zhang  12 , Shenyu Li  4 , Kexin Wang  9 , Guangling Wang  4 , Wanqiu Zhou  4 , Jianfeng Liu  9 , Cong Yu  10 , Shuzhen Zhang  4 , Liangtang Chang  4 , Dafina Toplanaj  5 , Mengni Chen  4 , Jiabing Liu  4 , Yun Zhao  4 , Biyu Ren  4 , Hanyu Shi  4 , Hui Zhang  4 , Haotian Yan  4 , Jianyun Ma  2 , Lina Wang  4 , Yan Li  4 , Yichen Zuo  2 , Linjie Lu  4 , Liqin Gu  4 , Shuting Li  4 , Yaqian Wang  8 , Yinying He  4 , Shengkang Li  12 , Qi Zhang  4 , Yanbing Lu  4 , Yannong Dou  4 , Yuan Liu  4 , Anqi Zhao  13 , Minyuan Zhang  4 , Xinyan Zhang  4 , Ying Xia  4 , Wei Zhang  8 , Huateng Cao  4 , Zhiyue Lu  4 , Zixian Yu  4 , Xin Li  2 , Xiaofei Wang  4 , Zhifeng Liang  9 , Shengjin Xu  9 , Cirong Liu  9 , Changhong Zheng  13 , Chun Xu  9 , Zhiyong Liu  9 , Chengyu Li  3 , Yan-Gang Sun  9 , Xun Xu  14 , Colette Dehay  5 , Julien Vezoli  5 , Mu-Ming Poo  15 , Jianhua Yao  16 , Longqi Liu  17 , Wu Wei  18 , Henry Kennedy  19 , Zhiming Shen  20
Affiliations
Free article

Single-cell spatial transcriptome atlas and whole-brain connectivity of the macaque claustrum

Ying Lei et al. Cell. .
Free article

Abstract

Claustrum orchestrates brain functions via its connections with numerous brain regions, but its molecular and cellular organization remains unresolved. Single-nucleus RNA sequencing of 227,750 macaque claustral cells identified 48 transcriptome-defined cell types, with most glutamatergic neurons similar to deep-layer insular neurons. Comparison of macaque, marmoset, and mouse transcriptomes revealed macaque-specific cell types. Retrograde tracer injections at 67 cortical and 7 subcortical regions defined four distinct distribution zones of retrogradely labeled claustral neurons. Joint analysis of whole-brain connectivity and single-cell spatial transcriptome showed that these four zones containing distinct compositions of glutamatergic (but not GABAergic) cell types preferentially connected to specific brain regions with a strong ipsilateral bias. Several macaque-specific glutamatergic cell types in ventral vs. dorsal claustral zones selectively co-projected to two functionally related areas-entorhinal cortex and hippocampus vs. motor cortex and putamen, respectively. These data provide the basis for elucidating the neuronal organization underlying diverse claustral functions.

Keywords: cell type; claustrum; connectome; insula; monkey; non-human primates; spatial transcriptome; tract tracing.

PubMed Disclaimer

Conflict of interest statement

Declaration of interests L. Liu and X.X. are the co-inventors of Stereo-seq technology. The chip, procedure, and applications of Stereo-seq are covered in pending patents.

LinkOut - more resources