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Comment
. 2025 May;44(9):2397-2399.
doi: 10.1038/s44318-025-00419-y. Epub 2025 Apr 7.

Updated values for Table 1 of fastest rubisco carboxylation rates in Davidi et al 2020

Yi-Chin Candace Tsai  1 , Zhijun Guo  1 , Ron Milo  2 , Oliver Mueller-Cajar  3
Affiliations
Comment

Updated values for Table 1 of fastest rubisco carboxylation rates in Davidi et al 2020

Yi-Chin Candace Tsai et al. EMBO J. 2025 May.

Abstract

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Conflict of interest statement

Disclosure and competing interests statement. The authors declare no competing interests.

Figures

Figure 1
Figure 1. Comparison of the data points reported in Davidi et al (2020) after correcting for the factor two mistake (gray) and the newly collected dataset (black).
Filled symbols were measured using protein purified in 2019, and open symbols were acquired using newly purified enzymes.
Figure 2
Figure 2. Correlation between the kcat values obtained using the coupled spectrophotometric Rubisco assay and the radiometric assay.
Lower rates are expected for coupled spectrophotometric assays when compared to a direct assay, such as the radiometric discontinuous 14CO2 fixation assay used here because of substrate limitation in the coupled reactions (Sharwood et al, 2016).
See this image and copyright information in PMC

Comment on

References

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