Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review

Leukocyte Proliferation Assay (Immunostimulation): Version 2.1

Timothy M. Potter  1 , Edward Cedrone  1 , Sarah L. Skoczen  1 , Barry W. Neun  1 , Marina A. Dobrovolskaia  1
In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May. NCL Method ITA-6.2.
2024 Jul.
Affiliations
Free Books & Documents
Review

Leukocyte Proliferation Assay (Immunostimulation): Version 2.1

Timothy M. Potter et al.
Free Books & Documents

Excerpt

This document describes a protocol for assessing the effect of a nanoparticle formulation on the basic immunologic function of human lymphocytes, i.e. measurement of lymphocyte proliferative responses [1, 2]. This assay will allow for measurement of a nanoparticles’ ability to induce proliferative response of human lymphocytes. This document is identical to part A of the NCL protocol ITA-6.1 (formerly NCL ITA-6).

PubMed Disclaimer

Similar articles

  • Leukocyte Proliferation Assay: Version 2.
    Potter TM, Cedrone E, Skoczen SL, Neun BW, Dobrovolskaia MA. Potter TM, et al. 2020 May. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-6.2. 2020 May. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-6.2. PMID: 39013042 Free Books & Documents. Review.
  • Leukocyte Proliferation Assay: Version 2.
    Potter TM, Cedrone E, Skoczen SL, Neun BW, Dobrovolskaia MA. Potter TM, et al. 2020 May. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-6.3. 2020 May. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-6.3. PMID: 39013061 Free Books & Documents. Review.
  • Leukocyte Proliferation Assay (Immunosuppression): Version 2.1.
    Potter TM, Cedrone E, Skoczen SL, Neun BW, Dobrovolskaia MA. Potter TM, et al. 2024 Jul. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-6.3. 2024 Jul. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-6.3. PMID: 40198039 Free Books & Documents. Review.
  • Leukocyte Proliferation Assay (Immunostimulation and Immunosuppression): Version 4.2.
    Potter TM, Cedrone E, Skoczen SL, Neun BW, Dobrovolskaia MA. Potter TM, et al. 2024 Jul. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-6.1. 2024 Jul. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-6.1. PMID: 39999260 Free Books & Documents. Review.
  • Detection of nanoparticles’ ability to stimulate toll-like receptors using HEK-Blue reporter cell lines: Version 2.
    Cedrone E, Dobrovolskaia MA. Cedrone E, et al. 2023 Jan. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-29. 2023 Jan. In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May 1–. NCL Method ITA-29. PMID: 39013057 Free Books & Documents. Review.
See all similar articles

References

    1. Current Protocols in Immunology. Edited by: Coligan John E. (NIAID, NIH; ); Bierer Barbara (Brigham & Women’s Hospital; ); Margulies David H. (NIAID, NIH; ); Shevach Ethan M. (NIAID, NIH; ); Strober Warren (NIAID, NIH; ); Coico Richard (Weill Medical College of Cornell University; ); John Wiley & Sons, Inc., 2005.
    1. Standard practice for evaluation of immune responses in biocompatibility testing using ELISA tests, lymphocytes proliferation, and cell migration. ASTM F1906-98.
    1. Dobrovolskaia MA, McNeil SE. Understanding the correlation between in vitro and in vivo immunotoxicity tests for nanomedicines. J Control Release. 2013;172(2):456–66. - PMC - PubMed

LinkOut - more resources