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. 2025 Mar 24:2025:10.17912/micropub.biology.001523.
doi: 10.17912/micropub.biology.001523. eCollection 2025.

Identification of a new allele of catp-4

Anushree Gurjar  1 , Amber R Krauchunas  1
Affiliations

Identification of a new allele of catp-4

Anushree Gurjar et al. MicroPubl Biol. .

Abstract

CATP-4 is a subunit of a Na + /K + -ATPase important for sperm motility and fertility. Here, we report the characterization of a new allele of catp-4 isolated from a forward genetic screen for sterile mutants. The catp-4 ( as46 ) allele has a missense mutation that substitutes a highly conserved Glycine which lies close to an ATP binding site. This mutation results in a loss-of-function phenotype comparable to that of the catp-4 null alleles, emphasizing the importance of this residue for CATP-4 function. This is for the first time that a phenotype has been reported for a single missense mutation in the catp-4 gene.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. Phenotypic characterization of <i>as46</i> and its causative mutation
Figure 1. Phenotypic characterization of as46 and its causative mutation
A. Hermaphrodite self-fertility is significantly reduced compared to wild-type N2 hermaphrodites (p<0.0001). B. catp-4 ( as46 ); him-5 male fertility is significantly reduced compared to wild-type N2 males when mated to fog-2 ( oz40 ) hermaphrodites (p<0.0001). Each dot on the graph represents the total number of progeny produced by one individual hermaphrodite (n≥10). Error bars represent standard error (SEM). C. DAPI staining of catp-4 ( as46 ) and wild-type N2 hermaphrodites at L4+6 hrs L4+24 hrs. The spermathecae are marked by circles. Presence of sperm in the uterus (indicated by an arrow) is only observed in catp-4 ( as46 ) hermaphrodites. Scale bar = 20µm D. Morphology of spermatids and activated spermatozoa from him-5 ( e1490 ) control males and catp-4 ( as46 ); him-5 ( e1490 ) males. Scale bar = 10µm E. Aspect ratio comparison of him-5 ( e1490 ) sperm and catp-4 ( as46 ); him-5 ( e1490 ) sperm (n=140). Error bars represent standard error (SEM). F. Schematic of the catp-4 genomic locus indicating the location of the as46 mutation in the fourth exon. Below is an alignment of C. elegans CATP-4 with Na + /K + -ATPases from other organisms. The conserved glycine residue that is changed from glycine (G) to arginine (R) in the catp-4 ( as46 ) mutant is marked with a black rectangle.
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References

    1. Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974 May 1;77(1):71–94. doi: 10.1093/genetics/77.1.71. - DOI - PMC - PubMed
    1. Clarke Erlyana K., Rivera Gomez Katherine A., Mustachi Zaki, Murph Mikaela C., Schvarzstein Mara. Manipulation of Ploidy in <em>Caenorhabditis elegans</em>. Journal of Visualized Experiments. 2018 Mar 15;(133) doi: 10.3791/57296. - DOI - PMC - PubMed
    1. Doitsidou Maria, Poole Richard J., Sarin Sumeet, Bigelow Henry, Hobert Oliver. C. elegans Mutant Identification with a One-Step Whole-Genome-Sequencing and SNP Mapping Strategy. PLoS ONE. 2010 Nov 8;5(11):e15435–e15435. doi: 10.1371/journal.pone.0015435. - DOI - PMC - PubMed
    1. Hansen Jody M, Chavez Daniela R, Stanfield Gillian M. COMP-1 promotes competitive advantage of nematode sperm. eLife. 2015 Mar 19;4 doi: 10.7554/elife.05423. - DOI - PMC - PubMed
    1. Jaramillo-Lambert Aimee, Fuchsman Abigail S, Fabritius Amy S, Smith Harold E, Golden Andy. Rapid and Efficient Identification of Caenorhabditis elegans Legacy Mutations Using Hawaiian SNP-Based Mapping and Whole-Genome Sequencing . G3 Genes|Genomes|Genetics. 2015 May 1;5(5):1007–1019. doi: 10.1534/g3.115.017038. - DOI - PMC - PubMed

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