Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025:2897:147-157.
doi: 10.1007/978-1-0716-4406-5_10.

Cryopreservation of Dog Semen

Jesús Dorado  1 , Isabel Ortiz  2
Affiliations

Cryopreservation of Dog Semen

Jesús Dorado et al. Methods Mol Biol. 2025.

Abstract

Cryopreservation of dog semen is an assisted reproductive technology of the utmost importance for increasing the genetic pool and developing the dog breeding industry. This technique is not unharmful for the sperm, and factors such as extender, cryoprotectant, and cooling-warming rates have been shown to affect the after-thawing quality of canine sperm.This chapter aims to describe the current range of methodologies available that are key to ensure successful freezing and thawing of dog sperm.

Keywords: Canine; Cryobank; Extender; Freezing; Spermatozoa; Tris-based extender.

PubMed Disclaimer

References

    1. Colombo M, Morselli MG, Franchi G, Schäfer-Somi S, Luvoni GC (2022) Freezability of dog semen after collection in field conditions and cooled transport. Animals 12(7). https://doi.org/10.3390/ani12070816
    1. Peña FJ, Núñez-Martínez I, Morán JM (2006) Semen technologies in dog breeding: an update. Reproduction in domestic animals =. Zuchthygiene 41(Suppl 2):21–29. https://doi.org/10.1111/j.1439-0531.2006.00766.x - DOI - PubMed
    1. Farstad W (2000) Current state in biotechnology in canine and feline reproduction. Anim Reprod Sci 60–61:375–387. https://doi.org/10.1016/s0378-4320(00)00106-8 - DOI - PubMed
    1. Kim S, Lee Y, Yang H, Kim Y-J (2012) Rapid freezing without cooling equilibration in canine sperm. Anim Reprod Sci 130(1):111–118. https://doi.org/10.1016/j.anireprosci.2011.12.014 - DOI - PubMed
    1. Qamar AY, Fang X, Kim MJ, Cho J (2019) Myoinositol supplementation of freezing medium improves the quality-related parameters of dog sperm. Animals 9(12). https://doi.org/10.3390/ani9121038

Substances

LinkOut - more resources