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. 2025:2897:183-192.
doi: 10.1007/978-1-0716-4406-5_13.

Sperm Cryopreservation in Boars

Inmaculada Parrilla  1 , María A Gil  2 , Cristina Cuello  2 , Emilio A Martinez  2
Affiliations

Sperm Cryopreservation in Boars

Inmaculada Parrilla et al. Methods Mol Biol. 2025.

Abstract

Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term preservation of boar fertility. Here, we describe a two-step cryopreservation protocol for boar sperm using lactose-egg yolk and glycerol-based extenders.

Keywords: Boar; Cooling; Cryoprotectants; Freezing; Spermatozoa; Thawing.

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References

    1. Yeste M (2015) State-of-the-art of boar sperm preservation in liquid and frozen state. Anim Reprod 14:69–81. https://doi.org/10.21451/1984-3143-AR895 - DOI
    1. Whaley D, Damyar K, Witek RP et al (2021) Cryopreservation. An overview of principles and cell-specific considerations. Cell Transplant 30:1–12. https://doi.org/10.1177/0963689721999617 - DOI
    1. Bailey JL, Lessard C, Jacques J et al (2008) Cryopreservation of boar semen and its future importance to the industry. Theriogenology 70:1251–1259. https://doi.org/10.1016/j.theriogenology.2008.06.014 - DOI - PubMed
    1. Rocha LGP, Zangeronimo MG, Murgas LDS et al (2015) Evaluation of two different boar semen freezing protocols and their effects on semen quality after thawing. Anim Reprod 12:871–875
    1. Parrilla I, del Olmo D, Sijses L et al (2012) Differences in the ability of spermatozoa from individual boar ejaculates to withstand different semen-processing techniques. Anim Reprod Sci 132:66–73. https://doi.org/10.1016/j.anireprosci.2012.04.003 - DOI - PubMed

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