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. 2025:2897:193-206.
doi: 10.1007/978-1-0716-4406-5_14.

Cryopreservation of Horse Sperm

Jordi Miró  1 , Iris Martínez-Rodero  2 , Marc Yeste  3   4   5 , Jaime Catalán  2   3   4
Affiliations

Cryopreservation of Horse Sperm

Jordi Miró et al. Methods Mol Biol. 2025.

Abstract

Cryopreservation is currently the only strategy for long-term conservation of equine sperm. To get optimal post-thaw sperm survival, carefully following each step of the freezing protocol is crucial. First, one needs to obtain and exhaustively analyze an ejaculate of good sperm quality. Then, the seminal plasma is removed by centrifugation, and the resulting pellet is resuspended in a certain volume of the freezing medium to reach the right sperm concentration. Finally, sperm samples are packaged into 0.5-mL straws, cooled, and frozen using an automatic, controlled-rate freezer. Once the temperature reaches -120 °C, straws are plunged into liquid nitrogen and stored in tanks until their further use.

Keywords: Cryopreservation; Freezing media; Horse; Semen analysis; Sperm.

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References

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