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. 2025 Apr 9;15(1):12167.
doi: 10.1038/s41598-025-85439-8.

Regulation of FOXL2 gene in ovarian granulosa cell tumor by JNK inhibitor

Yuzhu Zhang #  1 , Yu Wang #  2 , Yuan Gu  2 , Yang Liu  2 , Guohua Liu  2 , Jun Wu  3 , Nan Bai  4
Affiliations

Regulation of FOXL2 gene in ovarian granulosa cell tumor by JNK inhibitor

Yuzhu Zhang et al. Sci Rep. .

Abstract

To explore the regulatory effect of c-Jun N-terminal kinase (JNK) inhibitor (SP600125) on forkhead box protein L2 (FOXL2) gene in human ovarian granulosa cell tumor cells (KGN cells). The main pathogenic gene FOXL2 of ovarian cancer was screened by bioinformatics method. KGN cells were randomly divided into control group and experimental group. Different concentrations of SP600125 (0.1, 1, 5, 10, 50 µM) were added to the experimental group, and an equal volume of dimethyl sulfoxide (DMSO) was added to the control group. The cells were incubated for 48 h. Cell RNA was extracted and reverse transcribed into cDNA. The mRNA expression level of FOXL2 was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Proteins were extracted, and the expression level of FOXL2 protein was detected by Western blot. The proliferation ability of KGN cells treated with SP600125 was detected by MTT assay. Cell scratch assay was used to detect its migration ability. Different concentrations of JNK inhibitor reduced the expression of FOXL2 in ovarian granulosa cells KGN, and 1 µM had the best inhibitory effect. JNK inhibitor reduces the expression of FOXL2 in ovarian granulosa cell tumor KGN.

Keywords: FOXL2; JNK; KGN; Ovarian granulosa cells.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Screening of differentially expressed genes in ovarian cancer. Dataset GSE34526 from gene expression omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/). Using the GEO query package in R version 4.3.2.
Fig. 2
Fig. 2
The gene sequence and protein structural domains of FOXL2.
Fig. 3
Fig. 3
Results of total mRNA 1% agarose gel electrophoresis in twelve groups of cells. 1–6 and 7–12 represented GAPDH and FOXL2 in DMSO, co-clutured with 100 nM, 1, 5, 10, 50 μM JNK inhibitor group respectively. Number 13 refers to the DNA marker (TIANGEN).
Fig. 4
Fig. 4
After treatment with the JNK inhibitor, the mRNA expression levels of FOLX2 were detected in KGN cells by qRT-PCR. (*p < 0.05; **p < 0.01).
Fig. 5
Fig. 5
Protein expression levels of FOXL2 after treatment with JNK inhibitor.
Fig. 6
Fig. 6
Cell scratch assay.
Fig. 7
Fig. 7
The proliferation of KGN at different time periods.
See this image and copyright information in PMC

References

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