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. 2025 Jun;211(3):727-741.
doi: 10.1007/s10549-025-07695-6. Epub 2025 Apr 9.

A potential therapeutic molecule target: lncRNA AK023507 inhibits the metastasis of breast cancer by regulating the WNT/DOCK4/β-catenin axis

Biyu Diao #  1 , Yangjun Cai #  1   2 , Dandan Song #  1 , Yingying Hu #  1 , Bojian Xie  2 , Yang Kan  2 , Xiaoqu Hu  3
Affiliations

A potential therapeutic molecule target: lncRNA AK023507 inhibits the metastasis of breast cancer by regulating the WNT/DOCK4/β-catenin axis

Biyu Diao et al. Breast Cancer Res Treat. 2025 Jun.

Abstract

Purpose: Breast cancer (BC) has become the most common malignant tumor in women worldwide. This study was carried out to find and validate a novel molecular therapeutic target for BC.

Methods: Long non-coding RNA (lncRNA) AK023507 was selected as the study objects through microarray analysis. The function of lncRNA AK023507 was verified by various cell function experiments in vitro, subcutaneous tumorigenesis experiments, and lung metastasis model experiments in vivo. The RNA pull-down experiment and Western blot experiment were used to confirm the mechanism regulation pathway and the recovery experiment was used to verify it. TCGA datasets were used for clinical and immune function prediction analysis.

Results: In vitro cell function tests and in vivo experiments suggested that overexpression of lncRNA AK023507 inhibited the proliferation and metastasis of BC cells. The RNA pull-down experiment and Western blot analysis validated that lncRNA AK023507 interacted with the dedicator of cytokinesis 4 (DOCK4) protein. Analysis of public databases predicted that DOCK4 is a potential prognostic risk factor associated with epithelial-mesenchymal transition (EMT) and central memory T cell (TCM) cellular immune infiltration.

Conclusions: LncRNA AK023507 inhibits the proliferation and metastasis of BC by regulating the DOCK4/β-catenin axis. This discovery will provide new potential therapeutic targets for BC.

Keywords: Breast cancer; DOCK4; LncRNA AK023507; Metastasis; β-Catenin.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests. Ethics statement: Ethics approval for this study was obtained from the Ethics Committee of the First Affiliated Hospital of Wenzhou Medical University. Ethical approval: This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Wenzhou Medical University (No.wydw2019 - 0496). Consent for participate: Not applicable. Consent for publish: Not applicable.

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References

    1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A et al (2021) Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA 71:209–249 - PubMed
    1. Cai Z, Liu Q (2019) Understanding the Global Cancer Statistics 2018: implications for cancer control. Sci China Life Sci 64:1017–1020 - PubMed - DOI
    1. Azamjah N, Soltan-Zadeh Y, Zayeri F (2019) Global trend of breast cancer mortality rate: a 25-year study. Asian Pac J Cancer Prev 20(7):2015–2020 - PubMed - PMC - DOI
    1. Scully OJ, Bay BH, Yip G, Yu Y (2012) Breast cancer metastasis. Cancer Genomics Proteomics 9(5):311–320 - PubMed
    1. Liu Y, Sharma S, Watabe K (2015) Roles of lncRNA in breast cancer. Front Biosci (Schol Ed) 7(1):94–108 - PubMed - DOI

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