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Review
. 2025 Sep 1:262:116878.
doi: 10.1016/j.jpba.2025.116878. Epub 2025 Apr 7.

Novel approaches in CRISPR/Cas12a-based sensing for HCC diagnosis - A review (2020-2025)

Cunhong Cen  1 , Xiyu Liu  1 , Wei He  1 , Xiaohong Tan  2 , Guiyin Li  3 , Na Jintong  4
Affiliations
Review

Novel approaches in CRISPR/Cas12a-based sensing for HCC diagnosis - A review (2020-2025)

Cunhong Cen et al. J Pharm Biomed Anal. .

Abstract

Early diagnosis of hepatocellular carcinoma (HCC) is crucial for improving patient survival and treatment outcomes and the early detection of biomarkers for HCC is key to achieving this goal. However, conventional detection methods often lack sufficient specificity and sensitivity. In recent years, CRISPR/Cas12a-based biosensing has gained significant attention due to its ease of use and high sensitivity, demonstrating its potential to address the limitations of conventional detection methods. This paper primarily reviews the research progress of CRISPR/Cas12a-based biosensors for HCC detection, introducing their fluorescence, electrochemical, colorimetric, and other detection principles, as well as practical applications in detail. Additionally, the differences in sensitivity, specificity, and detection speed among different types of CRISPR/Cas12a biosensors are comparatively analyzed. Finally, the potential future directions for the development and application of CRISPR/Cas12a technology in clinical settings are explored.

Keywords: Biosensing; CRISPR/Cas12a; Hepatocellular carcinoma; Nucleic acid detection; Protein detection.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no conflict of interest or personal relationships that could have appeared to influence the work reported in this paper. The authors guarantee that the work is an original work because it has never been published before and has not been submitted to other places for publication. We all agree to submit and publish our paper entitled "Novel Approaches in CRISPR/Cas12a-Based Sensing for HCC Diagnosis-A Review (2020–2025)" in Your Journal.

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